Endophytic Fungal Diversity in the Needles and Shoots Infected with Dieback of Larix olgensis

被引：0
作者：
Xian, Xie [1 ,2 ]
Xun, Deng [3 ]
Liwen, Song [4 ]
Jun, Liang [2 ]
机构：
[1] Agriculture Science and Engineering School, Liaocheng University, Liaocheng
[2] Key Laboratory of Forestry Ecology and Environment of National Forestry and Grassland Administration Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing
[3] Heilongjiang Forest Protection Research Institute, Heilongjiang
[4] Jilin Academy of Forestry Science, Changchun
来源：
Linye Kexue/Scientia Silvae Sinicae | 2024年 / 60卷 / 10期
关键词：
Botryosphaeria laricina; community structure; endophytic fungi; needles; shoots;
D O I：
10.11707/j.1001-7488.LYKX20230386
中图分类号：
学科分类号：
摘要：
【Objective】This study aims to investigate the relationship between the differences in the diversity and community structure of endophytic fungi in the needles and shoots of Larix olgensis (larch) infected by Botryosphaeria laricina, and the incidence of larch dieback, so as to provide reference for the prevention of larch dieback.【Method】Based on high-throughput sequencing technology, the differences in community structure, diversity and functional nutrient types of endophytic fungi between asymptomatic and diseased larch needles and shoots were analyzed by means of OTU number, identification group, diversity index and FUNGuild prediction.【Result】The sequencing results showed that there were 1 091 OTUs in needle samples and 1 083 OTUs in shoot samples. The diversity of endophytic fungi in asymptomatic needles was significantly higher than that in the heavily infected needles, and there was no significant difference in endophytic fungal diversity between the asymptomatic and infected shoots. The dominant fungal phylum in needles and shoots was Ascomycetes, and the dominant order and genus in asymptomatic needles were Chaetothyriales and Arthrocatena, respectively. After infection with by B. laricina, the dominant order and genus in needles were Botryosphaeriales and Neofusicoccum, respectively, while Capnodiales and Microcyclosporella were the dominant fungal order and genus in shoots, respectively. LefSe analysis indicated that there were a total of 54 significant differential groups in fungal communities in needles and shoots. PCoA analysis further confirmed that the endophytic fungal communities of asymptomatic needles were significantly different from those of infected needles (anosim R=0.695, P < 0.05). The fungal nutritional types in needles were mainly pathological and saprophytic, and the pathological fungi were dominated after infection. The nutritional types of fungi in the shoots were mainly pathological and saprophytic.【Conclusion】Compared with the shoots of L .olgensis, the endophytic fungal community structure in needle changes more significantly after infection by B. laricina, and the pathogen is enriched in the needles, thereby promoting the development of larch dieback. © 2024 Chinese Society of Forestry. All rights reserved.
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页码：76 / 85
页数：9
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