Biochar enhances Cd mineralization through microbially induced carbonate precipitation as a soil remediation strategy for rice paddies

Microbial induced carbonate precipitation (MICP) is a promising technique for remediating Cd-contaminated soils. However, the high cost and potential disruption to soil micro-ecology due to the excessive urea addition remain significant challenges, limiting the broader application of MICP technology in agricultural soils. This study aims to improve the efficiency of Cd immobilization by MICP under low urea levels by investigating the stimulatory effect of porous materials on urease secretion by ureolytic bacteria. Results demonstrate that these materials, including biochar, activated carbon, zeolite, and oyster shell, can stimulate the growth of ureolytic bacteria strain kp-22, but not diatomite. Urease activity was greatly improved within 12 h, and the Cd removal rate reached over 82.12% within 0.5 h. Notably, biochar supported urealytic bacterium strain kp-22 (BCM) can steadily remove Cd in solution, with the Cd removal rate remaining close to 99% even after multiple additions of Cd. XRD analysis shows that Cd was removed by BCM due to the formation of CdCO3. Soil experiment reveals that BCM significantly decreased the bioavailable Cd content in both flooded and unflooded paddy soils, even when the urea addition was at a dosage suitable for agricultural production. 16S rRNA gene sequencing shows that the disturbance caused by BCM to the soil bacterial community was lower than that caused by strain kp-22 alone. These findings offer new insights into enhancing the efficiency of MICP for Cd remediation, increasing the potential for broader application of MICP technology in sustainable agriculture. © 2024 Elsevier Ltd