Differential susceptibility to hypoxia in hypoxia-inducible factor 1-alpha (HIF-1α)-targeted freshwater water flea Daphnia magna mutants

被引：3

作者：

Lee, Yoseop ^{[1
]}

Byeon, Eunjin ^{[1
]}

Lee, Jin-Sol ^{[1
]}

Maszczyk, Piotr ^{[2
]}

Kim, Hyung Sik ^{[3
]}

Sayed, Alaa El-Din H. ^{[4
]}

Yang, Zhou ^{[5
]}

Lee, Jae-Seong ^{[1
]}

Kim, Duck-Hyun ^{[1
]}

机构：

[1] Sungkyunkwan Univ, Coll Sci, Dept Biol Sci, Suwon 16419, South Korea

[2] Univ Warsaw, Fac Biol, Dept Hydrobiol, Warsaw, Poland

[3] Sungkyunkwan Univ, Sch Pharm, Suwon 16419, South Korea

[4] Assiut Univ, Fac Sci, Dept Zool, Assiut 71516, Egypt

[5] Nanjing Normal Univ, Sch Biol Sci, Jiangsu Key Lab Biodivers & Biotechnol, Nanjing 210023, Peoples R China

来源：

MARINE POLLUTION BULLETIN | 2024年 / 209卷

基金：

新加坡国家研究基金会;

关键词：

Hypoxia; HIF-1; alpha; CRISPR/Cas9-mediated gene targeting; Daphnia magna; LIFE-SPAN; OXYGEN; TEMPERATURE; HIF-1-ALPHA; EXPRESSION; ROLES; HIF-2-ALPHA; MECHANISMS; HEMOGLOBIN; RESPONSES;

D O I：

10.1016/j.marpolbul.2024.117138

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The water flea, Daphnia magna, serves as a key model organism for investigating the response of aquatic organisms to environmental stressors, including hypoxia. Hypoxia-inducible factor 1-alpha (HIF-1 alpha) is a central regulatory protein involved in the cellular response to hypoxic conditions. In this study, we used CRISPR/Cas9 gene editing to create D. magna mutant lines with targeted alterations in the HIF-1 alpha gene. Mutants demonstrated decreased survival and reproductive output and down-regulated genes for the HIF-1 alpha-mediated pathway in low- oxygen conditions. These findings suggest that the HIF-1 alpha pathway is a critical component of resistance to hypoxia in D. magna. This study provides novel insights into the molecular basis of hypoxia tolerance of HIF-1 alpha in D. magna and expands our understanding of how aquatic organisms can adapt to or be challenged by changing oxygen levels in the face of global environmental changes.

引用

页数：10

共 84 条

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