Extensive Analysis of Mulberry (Morus rubra L.) Polysaccharides with Different Maturities by Using Two-Step Extraction and LC/QqQ-MS

被引：0

作者：

Zhu, Kai ^{[1
]}

Wu, Jinghua ^{[1
,2
]}

Hu, Ankai ^{[1
]}

Yin, Zihao ^{[1
]}

Hou, Zhiqiang ^{[1
]}

Ye, Xingqian ^{[1
,3
,4
,5
]}

Chen, Shiguo ^{[1
,3
,4
,5
]}

机构：

[1] College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Integrated Research Base of Southern Fruit and Vegetable Preserv

[2] College of Agriculture and Biotechnology, Zhejiang University, Hangzhou,310058, China

[3] Zhejiang University Zhongyuan Institute, Zhengzhou,450000, China

[4] Ningbo Research Institute of Zhejiang University, Ningbo,315100, China

[5] Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing,314102, China

来源：

Journal of Agricultural and Food Chemistry | 2023年

关键词：

Number:; 2022YFF1100200; Acronym:; NKRDPC; Sponsor: National Key Research and Development Program of China; -; 2022ZY001; ZJU; Sponsor: Zhejiang University; 100000-11320/207; Sponsor:;

D O I：

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学科分类号：

摘要：

A primary challenge of polysaccharide analysis is the need for comprehensive extraction and characterization methods. In this study, mulberry polysaccharides at different maturities were fully extracted through a two-step process involving ethylenediaminetetraacetic acid (EDTA) and sodium hydroxide (NaOH), and their structures were determined by a combination analysis of monosaccharides and glycosidic linkages based on liquid chromatography triple quadrupole mass spectrometry (LC/QqQ-MS). The results indicate mulberry polysaccharides mainly contain highly branched pectic polysaccharides, (1,3,6)-linked glucan, xylan, and xyloglucan, but the content of different portions varies at different maturity stages. HG decreases from 19.12 and 19.14% (green mulberry) to 9.80 and 6.08% (red mulberry) but increases to 17.83 and 11.83% as mulberry transitioned from red to black. In contrast, the contents of glucan showed opposite trends. When mulberry turns red to black, the RG-I arabinan chains decrease from 47.75 and 28.86% to 13.16 and 12.72%, while the galactan side chains increase from 1.18 and 1.91 to 8.3 and 6.49%, xylan and xyloglucan show an increase in content. Overall, the two-step extraction combined with LC/QqQ-MS provides a new strategy for extensive analysis of complex plant polysaccharides. © 2024 American Chemical Society.

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