Method for Rapid Detection of Bacteria Using Magnetic Nanoparticle Aggregates

被引：0

作者：

Pu Y. ^{[1
]}

Zhao H. ^{[1
,2
]}

Murayama T. ^{[3
]}

Tonthat L. ^{[1
,2
]}

Okita K. ^{[3
]}

Watanabe Y. ^{[3
]}

Yabukami S. ^{[1
,2
,3
]}

机构：

[1] School of Engineering, Tohoku Univ., 6-6-05 Aramaki aza-aoba, Aoba-ku, Sendai

[2] Graduate school of Engineering, Tohoku Univ., 6-6-05 Aramaki aza-aoba, Aoba-ku, Sendai

[3] Graduate school of Biomedical Engineering, Tohoku Univ., 6-6-05 Aramaki aza-aoba, Aoba-ku, Sendai

来源：

Journal of the Magnetics Society of Japan | 2023年 / 47卷 / 03期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

Antigen-antibody reaction; Bacteria detection; Fusobacterium nucleatum; Magnetic nanoparticle; Magnetic susceptibility;

D O I：

10.3379/msjmag.2305R003

中图分类号：

学科分类号：

摘要：

A novel method for the rapid detection of bacteria in the liquid phase for point-of-care testing was developed using magnetic nanoparticles (MNPs) conjugated with antibodies. We utilized the magnetic characteristic that the magnetic susceptibility decreases when the magnetism of MNP aggregate changes from ferromagnetism to superparamagnetism after bacteria bind to MNPs through an antigen-antibody reaction (Nanomag-D, 500 nmϕ, 0.2μl/sample). The magnetic susceptibility of Fusobacterium nucleatum samples was measured using a lab-made detection setup. We found that the susceptibility depends on the concentration of Fusobacterium nucleatum with optical density (OD) values ranging from 10-4 to 1 (i.e. 8 × 104 ~ 8 × 108 CFU/ml). In addition, the detection time was only about 49 seconds, which shows promise for point-of-care testing. © 2023 by the Magnetics Society of Japan.

引用

页码：66 / 69

页数：3

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