Effect of Liquid Nitrogen Freezing on Physiological and Biochemical Characteristics of Phyllostachys praecox Shoots during Frozen Storage

被引：0

作者：

Liao J. ^{[1
]}

Chen J. ^{[1
,2
]}

Xu L. ^{[1
]}

Lu H. ^{[1
,2
]}

Wang L. ^{[1
,2
]}

Jiao C. ^{[3
]}

机构：

[1] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan

[2] Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan

[3] Hubei Heyuan Gas Co. Ltd., Yichang

来源：

Shipin Kexue/Food Science | 2024年 / 45卷 / 13期

关键词：

freezing temperature; internal temperature; liquid nitrogen; Phyllostachys praecox shoots; physiological and biochemical characteristics;

D O I：

10.7506/spkx1002-6630-20231008-042

中图分类号：

学科分类号：

摘要：

Phyllostachys praecox shoots were frozen using liquid nitrogen at –60, –90 or –120 ℃ to an internal temperature of –18 ℃, or frozen at –90 ℃ to an internal temperature of –6, –12 or –18 ℃, vacuum-packed and stored in a freezer at –18 ℃ for 24 weeks. in order to analyze the effect of liquid nitrogen freezing on physiological and biochemical characteristics of P. praecox shoots during frozen storage, L-phenylalanine ammonialyase (pAL) and peroxidase (pOD) activities, total phenolic content, relative electrical conductivity, and water state were measured and ice crystal structure and cell morphology were observed. The results showed that with increasing freezing time, the pAL and pOD activities, total phenolic content, and peak area of free water in all six groups decreased significantly (P < 0.05), while the relative electrical conductivity increased significantly (P < 0.05), with the ice crystals and cells being deformed and damaged to varying degrees. Lower freezing temperature led to smaller ice crystals, lower pAL and pOD activities and relative electrical conductivity, higher total phenolic content, and better maintenance of cell morphology, but there was no significant difference in physiological and biochemical properties between P. praecox shoots frozen at –90 and –120 ℃ (P > 0.05). The pAL and pOD activities and relative conductivity of P. praecox shoots frozen at –6 ℃ were higher than those frozen at –12 and –18 ℃, and the size of ice crystals was smaller and the degree of cell damage was greater in P. praecox shoots frozen at –6 ℃ than at –12 and –18 ℃. The difference between P. praecox shoots frozen at –12 and –18 ℃ was not significant (P > 0.05). Collectively, these findings indicated that the most suitable liquid nitrogen freezing conditions of P. praecox shoots are –90 and –12 ℃ for freezing and internal temperature, respectively. © 2024 Chinese Chamber of Commerce. All rights reserved.

引用

页码：247 / 255

页数：8

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