Inhibitory Mechanism of A-type Oligomeric Proanthocyanidins from Abscising Litchi Fruits on Proinflammation Induced by Litchi Thaumatin-Like Protein

被引：0

作者：

Luo Y. ^{[1
]}

Zeng S. ^{[2
]}

Li L. ^{[2
]}

Xie C. ^{[2
]}

Yan J. ^{[3
]}

Li Y. ^{[3
]}

Luo Y. ^{[1
]}

Zhao L. ^{[1
,2
]}

机构：

[1] Guangxi Key Laboratory of Health Care Food Science and Technology, Hezhou University, Hezhou

[2] College of Food Science, South China Agricultural University, Guangzhou

[3] School of Life and Health Technology, Dongguan University of Technology, Dongguan

[4] Inkea Health Technology Co. Ltd., Dongguan

来源：

Shipin Kexue/Food Science | 2024年 / 45卷 / 08期

关键词：

A-type oligomeric proanthocyanidins; anti-inflammatory mechanism; litchi fruit; litchi thaumatin-like protein;

D O I：

10.7506/spkx1002-6630-20230708-074

中图分类号：

学科分类号：

摘要：

This study investigated the mechanism by which A-type oligomeric proanthocyanidins (A-OPC) from abscising litchi fruits inhibit proinflammation induced by litchi thaumatin-like protein (LcTLP). A-OPC were prepared by sequential ethanol extraction and purification by macroporous adsorption resin and preparative liquid chromatography. Structural analysis showed that A-OPC were mainly composed of proanthocyanidin A2, two A-type proanthocyanidin trimers and one A-type tetramer, with a purity of 88.69%. At 120 μg/mL concentration, A-OPC inhibited the secretion of nitric oxide (NO), interleukin (IL)-6 and tumor necrosis factor-α (TNF-α) by 58.38%, 39.80% and 86.31% in RAW264.7 cells treated with LcTLP, respectively (P < 0.01). Western blot showed that 120 μg/mL A-OPC reduced the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and significantly decreased the phosphorylation levels of p65, inhibitor of NF-κB (IκBα), c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38. Therefore, A-OPC down-regulated the phosphorylation levels of downstream proteins in the nuclear transcription factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, thereby suppressing LcTLP-induced inflammatory response. © 2024 Chinese Chamber of Commerce. All rights reserved.

引用

页码：145 / 152

页数：7

共 28 条

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