Lycium barbarum polysaccharides (LBP) suppresses hypoxia/reoxygenation (H/R)-induced rat H9C2 cardiomyocytes pyroptosis via Nrf2/HO-1 signaling pathway

被引：5
作者：
Gao, Yi [1 ]
Li, Huangen [2 ]
Que, Yongkang [1 ]
Chen, Weiwen [2 ]
Huang, Shi-Ying [3 ]
Liu, Wenjie [4 ]
Ye, Xiaotong [5 ]
机构：
[1] Huaqiao Univ, Dept Gen Med, Xiamen Changgeng Hosp, Xiamen 361000, Peoples R China
[2] Fujian Med Univ, Dept Crit Care Med, Quanzhou Hosp 1, Quanzhou 362000, Peoples R China
[3] Jimei Univ, Coll Ocean Food & Biol Engn, Xiamen 361000, Peoples R China
[4] Fujian Med Univ, Quanzhou Hosp 1, Dept Gen Med, Quanzhou 362000, Peoples R China
[5] Huaqiao Univ, Sch Med, Quanzhou 362000, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 280卷
关键词：
Lycium barbarum polysaccharides; Hypoxia/Reoxygenation (H/R); Nrf2/HO-1; Pyroptosis; INJURY; APOPTOSIS; MODEL; AXIS; AMPK;
D O I：
10.1016/j.ijbiomac.2024.135924
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This study aimed to explore the mechanism that Lycium barbarum polysaccharides (LBP) suppress hypoxia/reoxygenation (H/R)-caused pyroptosis in cardiomyocytes (H9C2) via the Nrf2/HO-1 pathway. Initially, we established the cell model of H/R (6 h hypoxia plus with 24 h reoxygenation), and found that 90 mu g/mL LBP was the optimal concentration. Subsequently, we confirmed that LBP reduced the apoptosis rate of cells after H/R, the activity of LDH, the inflammatory factors IL-1 beta and IL-18, and the levels of pyroptosis-specific markers ASC, NLRP3, and Caspase-1 (mRNAs and proteins). It increased the cell survival rate and the mRNA levels of the Nrf2/HO-1 pathway markers Nrf2 and HO-1, and allowed cytoplasmic Nrf2 protein to enter the nucleus to activate HO-1 protein. The Nrf2 siRNA2 caused the following events in H/R model: (1) the increases of the apoptosis rate, LDH activity, the levels of inflammatory factors (IL-1 beta and IL-18), the levels of ACS, NLRP3, and Caspase-1 (mRNAs and proteins); and (2) the decreases of the cell survival rate, the mRNA levels of Nrf2 and HO-1, and the protein levels of cytoplasm-Nrf2, nucleus-Nrf2, and HO-1. Therefore we concluded that 90 mu g/mL LBP suppressed H/R-induced H9C2 cardiomyocyte pyroptosis via the Nrf2/HO-1 pathway.
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页数：12
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