Deacetylation enhances the structure and gelation properties of konjac glucomannan/soy protein isolate cold-set gels

This study aimed to investigate the effect of the deacetylated konjac glucomannan (DKGM), with varying degree of deacetylation (DD), on the physicochemical and structural properties of transglutaminase-induced soy protein isolate (SPI) cold-set gels. Compared with native konjac glucomannan (KGM), DKGM significantly enhanced the gel strength, water-holding capacity, and thermal stability of the composite gels, with DK3 (DKGM with 65.85 % deacetylation) showing the most significant improvement. The secondary and tertiary structures of SPI in the DK3 group were the most stable. Compared with the KGM group, the DK3 group showed a 58.32 % increase in hydrophobic interaction and a 37.98 % decrease in free sulfhydryl content. The microstructure results demonstrated that DK3 was uniformly dispersed within the SPI network, promoting the formation of a continuous and dense network structure. This was mainly due to DK3 having a moderate particle size and low viscosity. Therefore, DKGM with a moderate DD is conducive to forming a more ordered and dense gel network structure, imparting optimal gel performance to the SPI cold-set gel.