A Versatile Platform for Point-of-Care Detection of Molecular Biomarkers

被引：1

作者：

Silva, Ines E. ^{[1
]}

Domingues, Cristiana ^{[1
]}

Chu, Virginia ^{[1
]}

Pedro Conde, Joao ^{[1
,2
]}

机构：

[1] Inst Engn Sistemas & Comp Microsistemas & Nanotecn, P-1000029 Lisbon, Portugal

[2] Univ Lisbon, Dept Bioengn, Inst Super Tecn, P-1049001 Lisbon, Portugal

来源：

IEEE SENSORS JOURNAL | 2024年 / 24卷 / 16期

关键词：

Microfluidics; Fluorescence; Photodiodes; Noise; Light emitting diodes; Biosensors; Performance evaluation; Abscisic acid (ABA); fluorescence detector; microfluidics; point-of-care testing (PoCT); INTEGRATED FLUORESCENCE DETECTION; MICROFLUIDIC SYSTEMS; CHIP; BIOMOLECULES;

D O I：

10.1109/JSEN.2024.3423678

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

This article presents a point-of-care testing (PoCT) molecular biomarker detection platform based on microfluidics technology. The core of the platform is composed of a miniaturized microfluidic component designed to perform immunoassays for molecular recognition and a fluorescence biosensor system, where the optical transducer is an hydrogenated amorphous silicon (a-Si:H) p-i-n photodiode, which converts the molecular recognition event into an electrical signal proportional to the quantity of emission from the fluorescence label tagged to the target molecule. The fluorescence biosensor system achieved a limit of detection (LOD) of 0.36 nM when measuring free Alexa430 fluorophore in an open microfluidic channel. The microfluidic device was able to successfully replicate the results of a laboratory benchtop immunoassay. As proof of concept, the platform was used to detect abscisic acid (ABA), a biomarker of grape maturation. The results showed a significantly different output for a green grape (low ABA levels) compared with a maturated grape (high ABA levels).

引用

页码：26388 / 26396

页数：9

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