Screening of glucosinolates degrading lactic acid bacteria and their utilization in rapeseed meal fermentation

被引:4
作者
Chen, Yifang [1 ]
Xie, Chong [1 ,2 ]
Bilal, Muhammad [1 ]
Wang, Pei [1 ,2 ,3 ]
Yang, Runqiang [1 ,2 ]
机构
[1] Nanjing Agr Univ, Coll Food Sci & Technol, Whole Grain Food Engn Res Ctr, Nanjing 210095, Jiangsu, Peoples R China
[2] Nanjing Agr Univ, Sanya Inst, Sanya 572024, Hainan, Peoples R China
[3] Nanjing Agr Univ, Natl Tech Innovat Ctr Reg Wheat Prod, Natl Engn & Technol Ctr Informat Agr, Key Lab Crop Physiol Ecol & Management,Minist Agr, Nanjing 210095, Jiangsu, Peoples R China
关键词
Rapeseed meal; Glucosinolates; Fermentation; Lactic acid bacteria; In vitro digestion; AMINO-ACIDS; FOOD; MYROSINASE; PROTEINS;
D O I
10.1016/j.gaost.2024.05.005
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Rapeseed meal is a promising food ingredient, but its utilization is limited by the presence of some potentially harmful ingredients, such as glucosinolates. Fermentation is a cost-effective method of detoxication but a foodgrade starter culture with glucosinolates degradation capacity is required. In this study, 46 strains of lactic acid bacteria from traditional paocai brines were screened for their ability to glucosinolate degradation. The results showed that more than 50% of the strains significantly degraded glucosinolates. Two strains of Lactiplantibacillus (p7 and s7) with high capacity of glucosinolates degradation through producing enzymes were identified. Then, an optimized condition for rapeseed meal fermentation by p7 was established to degrade glucosinolates, which can achieve about 80% degradation. UPLC/Q-TOF-MS analysis showed that the degradation rate of individual glucosinolates was different and the degradation rate of gluconapin and progoitrin in rapeseed meal can reach more than 90%. Meanwhile, fermentation with p7 can improve safety of rapeseed meal by inhibiting the growth of Enterobacteriaceae and improve its nutritional properties by degrading phytic acid. The in vitro digestion experiments showed that the content of glucosinolates in rapeseed meal decreased significantly during gastric digestion. Meanwhile, fermentation with p7 can greatly improve the release of soluble protein and increase the contents of free essential amino acids, such as lysine (increased by 12 folds) and methionine (increased by 10 folds).
引用
收藏
页码:168 / 176
页数:9
相关论文
共 44 条
[1]   Canola proteins: composition, extraction, functional properties, bioactivity, applications as a food ingredient and allergenicity - A practical and critical review [J].
Aider, Mohammed ;
Barbana, Chockry .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2011, 22 (01) :21-39
[2]   Discovery of a Bacterial Glycoside Hydrolase Family 3 (GH3) β-Glucosidase with Myrosinase Activity from a Citrobacter Strain Isolated from Soil [J].
Albaser, Abdulhadi ;
Kazana, Eleanna ;
Bennett, Mark H. ;
Cebeci, Fatma ;
Luang-In, Vijitra ;
Spanu, Pietro D. ;
Rossiter, John T. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016, 64 (07) :1520-1527
[3]  
Aljuobori A., 2014, Journal of Food Research, V3, P95
[4]   The effect of starter cultures on proteolytic changes and amino acid content in fermented sausages [J].
Aro, Juan Marcos Aro ;
Nyam-Osor, Purevdorj ;
Tsuji, Kayoko ;
Shimada, Ken-ichiro ;
Fukushima, Michihiro ;
Sekikawa, Mitsuo .
FOOD CHEMISTRY, 2010, 119 (01) :279-285
[5]   Large scale purification of rapeseed proteins (Brassica napus L.) [J].
Bérot, S ;
Compoint, JP ;
Larré, C ;
Malabat, C ;
Guéguen, J .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 818 (01) :35-42
[6]  
BOTTI MG, 1995, J BIOL CHEM, V270, P20530, DOI 10.1074/jbc.270.35.20530
[7]   Glucosinolates, structures and analysis in food [J].
Clarke, Don Brian .
ANALYTICAL METHODS, 2010, 2 (04) :310-325
[8]   Allergenic potential of novel proteins - What can we learn from animal production? [J].
Ekmay, Ricardo D. ;
Coon, Craig N. ;
Ladics, Gregory S. ;
Herman, Rod A. .
REGULATORY TOXICOLOGY AND PHARMACOLOGY, 2017, 89 :240-243
[9]   Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts [J].
Ferrero, Romina L. ;
Soto-Maldonado, Carmen ;
Weinstein-Oppenheimer, Caroline ;
Cabrera-Munoz, Zaida ;
Elvira Zuniga-Hansen, Maria .
FOODS, 2021, 10 (02)
[10]  
Folawiyo YL, 1996, J SCI FOOD AGR, V70, P241, DOI 10.1002/(SICI)1097-0010(199602)70:2<241::AID-JSFA490>3.0.CO