Screening of glucosinolates degrading lactic acid bacteria and their utilization in rapeseed meal fermentation

被引：0
作者：
Chen Y. [1 ]
Xie C. [1 ,2 ]
Bilal M. [1 ]
Wang P. [1 ,2 ,3 ]
Yang R. [1 ,2 ]
机构：
[1] College of Food Science and Technology, Whole Grain Food Engineering Research Center, Nanjing Agricultural University, Jiangsu, Nanjing
[2] The Sanya Institute of Nanjing Agricultural University, Hainan, Sanya
[3] National Technique Innovation Center for Regional Wheat Production/Key Laboratory of Crop Physiology, Ecology, and Management, Ministry of Agriculture/National Engineering and Technology Center for Information Agriculture, Nanjing Agricultural University,
来源：
Grain and Oil Science and Technology | 2024年 / 7卷 / 03期
关键词：
Fermentation; Glucosinolates; In vitro digestion; Lactic acid bacteria; Rapeseed meal;
D O I：
10.1016/j.gaost.2024.05.005
中图分类号：
学科分类号：
摘要：
Rapeseed meal is a promising food ingredient, but its utilization is limited by the presence of some potentially harmful ingredients, such as glucosinolates. Fermentation is a cost-effective method of detoxication but a food-grade starter culture with glucosinolates degradation capacity is required. In this study, 46 strains of lactic acid bacteria from traditional paocai brines were screened for their ability to glucosinolate degradation. The results showed that more than 50% of the strains significantly degraded glucosinolates. Two strains of Lactiplantibacillus (p7 and s7) with high capacity of glucosinolates degradation through producing enzymes were identified. Then, an optimized condition for rapeseed meal fermentation by p7 was established to degrade glucosinolates, which can achieve about 80% degradation. UPLC/Q-TOF-MS analysis showed that the degradation rate of individual glucosinolates was different and the degradation rate of gluconapin and progoitrin in rapeseed meal can reach more than 90%. Meanwhile, fermentation with p7 can improve safety of rapeseed meal by inhibiting the growth of Enterobacteriaceae and improve its nutritional properties by degrading phytic acid. The in vitro digestion experiments showed that the content of glucosinolates in rapeseed meal decreased significantly during gastric digestion. Meanwhile, fermentation with p7 can greatly improve the release of soluble protein and increase the contents of free essential amino acids, such as lysine (increased by 12 folds) and methionine (increased by 10 folds). © 2023 Elsevier B.V.
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页码：168 / 176
页数：8
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共 43 条
  • [1] Yadav B.P., Wagle P., Rasaily S., Et al., Evaluation on the role of different production factor on the yield of rapeseed, Int. J. Appl. Sci. Biotechnol., 10, pp. 225-227, (2022)
  • [2] Golebiewska K., Fras A., Golebiewski D., Rapeseed meal as a feed component in monogastric animal nutrition—a review, Ann. Anim. Sci., 22, pp. 1163-1183, (2022)
  • [3] Berot S., Compoint J.P., Larre C., Et al., Large scale purification of rapeseed proteins (Brassica napus L.), J. Chromatogr. B, 818, pp. 35-42, (2005)
  • [4] Szydlowska-Czerniak A., Amarowicz R., Szlyk E., Antioxidant capacity of rapeseed meal and rapeseed oils enriched with meal extract, Eur. J. Lipid Sci. Technol., 112, pp. 750-760, (2010)
  • [5] Niu Y., Rogiewicz A., Shi L., Et al., The effect of multi-carbohydrase preparations on non-starch polysaccharides degradation and growth performance of broiler chickens fed diets containing high inclusion level of canola meal, Anim. Feed Sci. Technol., 293, (2022)
  • [6] Wang Y., Cao K., Li H., Et al., Improvement of active peptide yield, antioxidant activity and anti-aging capacity of rapeseed meal fermented with yy-112 pure fermentation and co-fermentation, Food Biosci., 49, (2022)
  • [7] Marczak E.D., Usui H., Fujita H., Et al., New antihypertensive peptides isolated from rapeseed, Peptides, 24, pp. 791-798, (2003)
  • [8] He R., Ju X., Yuan J., Et al., Antioxidant activities of rapeseed peptides produced by solid state fermentation, Food Res. Int., 49, pp. 432-438, (2012)
  • [9] Ferrero R.L., Soto-Maldonado C., Weinstein-Oppenheimer C., Et al., Antiproliferative rapeseed defatted meal protein and their hydrolysates on mcf-7 breast cancer cells and human fibroblasts, Foods, 10, (2021)
  • [10] Konkol D., Popiela E., Skrzypczak D., Et al., Fermented rapeseed meal subjected to a biosorption process: a potential new feed additive with microelements for laying hens, Anim. Feed Sci. Technol., 308, (2024)
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