Effects of Storm Runoff on Bacterial Community Structure and Metabolic Activity and Water Quality in a Drinking Water Reservoir

To explore the influence of storm runoff on bacterial metabolic activity and water quality in Jinpen Reservoir，three-dimensional fluorescence spectroscopy parallel factor analysis，high-throughput DNA sequencing technology，and Biolog technology were used to analyze variations in the components of dissolved organic matter，bacterial community composition，and microbial metabolic activity during three periods（before，during，and one month after runoff）. The results showed that after storm runoff into the reservoir，the water temperature decreased，and dissolved oxygen in the middle of the water column increased significantly. Finally，the aerobic and low-temperature state in the middle water and anaerobic and low-temperature state in the bottom water was formed. Simultaneously，the concentration of total nitrogen，total phosphorus，organic matter，and turbidity increased significantly. After one month of runoff，the concentration of pollutant decreased，however，was still higher than that before runoff. Further，during the runoff period，the proportion of Proteobacteria and Bacteroidetes increased significantly and the sum of their relative abundance accounted for 63.6% of the bacterial community. The bacterial metabolic activity was also higher than that before. One month after runoff，the bacterial population structure did not recover and the bacterial metabolic activity still remained at a high level. © 2024 Science Press. All rights reserved.