Determination of Rosin in Meat and Their Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry

被引：0

作者：

Wanqin W. ^{[1
,2
]}

Feng J. ^{[1
,2
]}

Xiaolong F. ^{[1
,2
]}

Xiaoling Z. ^{[1
,2
]}

Kun H. ^{[1
,2
]}

Zhi H. ^{[1
,2
]}

Guojiao L. ^{[1
,2
]}

Yazhen Z. ^{[1
,2
]}

Songsong Z. ^{[1
,2
]}

Zhiyong F. ^{[1
,2
]}

Huixia W. ^{[1
,2
]}

机构：

[1] Hubei Provincial Institute for Food Supervision and Test, Wuhan

[2] Hubei Provincial Engineering andTechnology Research Center for Food Quality and Safety Test, Wuhan

来源：

Science and Technology of Food Industry | 2021年 / 42卷 / 12期

关键词：

dehydroabietic acid; high performance liquid chromatography-tandem mass spectrometry; meat; rosin; their products;

D O I：

10.13386/j.issn1002-0306.2020080257

中图分类号：

学科分类号：

摘要：

To establish a method for the determination of rosin in meat and their products by using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), the sample was extracted by ultrasonic instrument with acetonitrile. The extracts were then purified through solid-phase extraction (SPE) with C18 cartridges. The target of dehydroabietic acid was analyzed by HPLC-MS/MS, and quantified by internal standard method. The separation was performed on a Waters ACQUITY HSS T3 column (2.1 mm×100 mm, 1.8 μm) with isometric elution using 0.1%(v/v)formic acid aqueous solution-acetonitrile (30:70) as the mobile phase.The analysis of dehydroabietic acid was detected under electrospray positive ionization mode and the multiple reaction monitoring(MRM) mode. The dehydroabietic acid demonstrated good linearity in the range of 5~400 ng/mL, with the correlation coefficient values (r) being higher than 0.99. The limits of detection (LODs) and limits of quantification (LOQs) were found to be 80 and 200 μg/kg. The recoveries at three spiked levels (200, 400 and 2000 μg/kg) in blank matrix were in the range of 96.05%~104.17% with the relative standard deviations between 0.62%~3.68% (n=6). The analysis results of actual samples showed that the number of positive samples of dehydroabietic acid accounted for 16.67% of the total samples, and the residual content was in the range of 390.15~1850.03 μg/kg. The method was convenient, rapid, accurate and efficient. It could be employed for accurate determination of rosin in meat and their products. © 2021 Science and Technology of Food Industry. All rights reserved.

引用

页码：279 / 286

页数：7

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