Minimally invasive current-controlled electrical stimulation system for bacteria using highly capacitive conducting polymer-modified electrodes

被引：2
作者：
Makino D. [1 ]
Ueki A. [1 ]
Matsumoto H. [2 ]
Nagamine K. [1 ,2 ]
机构：
[1] Graduate School of Organic Materials Science, Yamagata University, 4-3-16 Jonan, Yamagata 992-8510, Yonezawa
[2] Faculty of Engineering, Department of Polymeric and Organic Materials Engineering, Yamagata University, 4-3-16 Jonan, Yamagata 992-8510, Yonezawa
来源：
Bioelectrochemistry | 2023年 / 149卷
关键词：
Bacteria; Electrical stimulation; Membrane potential; PEDOT;
D O I：
10.1016/j.bioelechem.2022.108290
中图分类号：
学科分类号：
摘要：
This paper proposes a minimally invasive current-controlled electric stimulation system based on a poly(3,4-ethylenedioxythiophene) (PEDOT)-modified electrode to characterize the dynamics of the membrane potential in Bacillus subtilis. A highly capacitive PEDOT-modified electrode enabled the injection of a large ionic charge to the surface of the cells suppressing cytotoxic pH change in the vicinity of the electrode. The current pulse induced a hyperpolarization response in B. subtilis around the electrode. Using quantitative charge injection through current-controlled electrical stimulation, the threshold charge density to excite B. subtilis was roughly estimated to be 530.8 µC cm−2 (of electrode surface area) for the first time. Our results provide the minimum electrical stimulation conditions necessary to minimal invasively control the bacterial membrane potential. © 2022 Elsevier B.V.
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