High-Throughput Absorbance-Activated Droplet Sorting for Engineering Aldehyde Dehydrogenases

被引:0
作者
Jain, Ankit [1 ]
Teshima, Mariko [2 ]
Buryska, Tomas [1 ]
Romeis, Dennis [2 ]
Haslbeck, Magdalena [2 ]
Doering, Manuel [2 ]
Sieber, Volker [2 ,3 ,4 ,5 ]
Stavrakis, Stavros [1 ]
de Mello, Andrew [1 ]
机构
[1] ETH, Inst Chem & Bioengn, Dept Chem & Appl Biosci, Vladimir Prelog Weg 1, CH-8093 Zurich, Switzerland
[2] Tech Univ Munich, Chem Biogenic Resources, Campus Straubing Biotechnol & Sustainabil, Schulgasse 16, D-94315 Straubing, Germany
[3] Tech Univ Munich, Catalyt Res Ctr, Ernst Otto Fischer Str 1, D-85748 Garching, Germany
[4] Univ Queensland, Sch Chem & Mol Biosci, 68 Copper Rd, St Lucia, Qld 4072, Australia
[5] Tech Univ Munich, SynBioFoundryTUM, Schulgasse 22, D-94315 Straubing, Germany
基金
瑞士国家科学基金会;
关键词
ENZYME; SEARCH;
D O I
10.1002/anie.202409610
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Recent decades have seen a dramatic increase in the commercial use of biocatalysts, transitioning from energy-intensive traditional chemistries to more sustainable methods. Current enzyme engineering techniques, such as directed evolution, require the generation and testing of large mutant libraries to identify optimized variants. Unfortunately, conventional screening methods are unable to screen such large libraries in a robust and timely manner. Droplet-based microfluidic systems have emerged as a powerful high-throughput tool for library screening at kilohertz rates. Unfortunately, almost all reported systems are based on fluorescence detection, restricting their use to a limited number of enzyme types that naturally convert fluorogenic substrates or require the use of surrogate substrates. To expand the range of enzymes amenable to evolution using droplet-based microfluidic systems, we present an absorbance-activated droplet sorter that allows droplet sorting at kilohertz rates without the need for optical monitoring of the microfluidic system. To demonstrate the utility of the sorter, we rapidly screen a 105-member aldehyde dehydrogenase library towards D-glyceraldehyde using a NADH mediated coupled assay that generates WST-1 formazan as the colorimetric product. We successfully identify a variant with a 51 % improvement in catalytic efficiency and a significant increase in overall activity across a broad substrate spectrum.
引用
收藏
页数:9
相关论文
empty
未找到相关数据