A calcium delivery system fabricated by poly-γ-glutamic acid: Preparation, characterization, and delivery mechanism studies

被引:0
作者
Lei, Peng [1 ]
Ma, Yuhang [1 ]
Xiao, Wei [1 ]
Wang, Linhao [1 ]
Fu, Heng [1 ]
Sun, Liang [1 ]
Qiu, Yibin [1 ]
Gu, Yian [1 ]
Zhang, Yatao [2 ]
Xu, Hong [1 ]
Wang, Rui [1 ]
机构
[1] Nanjing Tech Univ, Coll Food Sci & Light Ind, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Peoples R China
[2] Nanjing Shineking Biotech Co Ltd, Nanjing 211816, Peoples R China
关键词
Poly-gamma-glutamic acid; Chelated calcium; Calcium delivery system; Calcium bioavailability; WHEY-PROTEIN HYDROLYSATE; BINDING; PEPTIDE; ABSORPTION; BIOAVAILABILITY;
D O I
10.1016/j.lwt.2024.116846
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Calcium has limited bioavailability owing to the formation of calcium phosphate deposits in the gastrointestinal tract. In this study, a polyglutamic acid (PGA)-Ca complex of calcium chelate was prepared using gamma-PGA. The binding constant between gamma-PGA and calcium was determined to be 6.50 +/- 2.47 x 104 mol/L, where 3.6 units of glutamate was capable of binding one Ca2+. The structure of PGA-Ca was characterized, revealing that Ca2+ chelation promoted the aggregation of gamma-PGA molecules, disrupting the network structure and forming a mineralized beta-sheet-rich structure. In vitro digestion experiments demonstrated that PGA-Ca better maintained the soluble state of Ca2+ in the intestine compared with CaCl2. Cell absorption experiments showed that PGA-Ca exhibited 20% higher permeability through Caco-2 cell monolayers than CaCl2, indicating its higher bioavailability. Notably, PGA-Ca demonstrated improved absorption in the presence of dietary inhibitors, such as oxalate, tannin, and phytate, which compete with Ca2+. Finally, the molecular mechanism underlying the promotion of calcium absorption by PGA-Ca was investigated using RNA sequencing. The results reveal that PGA-Ca enhanced Ca2+ active transport by upregulating CACNA2D3 and downregulating CBARP, while enhancing paracellular Ca2+ transport by downregulating JAM2. This comprehensive study highlights the potential of PGACa as a highly beneficial calcium-delivery system.
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页数:11
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