Diagnostic Value of Multiplex Polymerase Chain Reaction in Detection of Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from Sepsis in Pediatrics

被引：2
作者：
Galeb S. [1 ]
Zaki M.E.S. [1 ]
Shrief R. [2 ]
Hassan R. [3 ]
Rizk M. [1 ]
机构：
[1] Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura
[2] Medical Microbiology and Immunology Department, Faculty of Medicine, Damietta University, New Damietta
[3] Pediatric Department, Faculty of Medicine, Mansoura University, Mansoura
来源：
Recent Patents on Biotechnology | 2021年 / 15卷 / 03期
关键词：
Acinetobacter baumannii; bacteria; PCR; Pseudomonas aeruginosa; sepsis; Stenotrophomonas maltophilia;
D O I：
10.2174/1872208315666210719104623
中图分类号：
学科分类号：
摘要：
Background: Proper identification of the causative organism in pediatric sepsis is crucial for early diagnosis and prevention of septic shock and organ failure. Objectives: The aim of the present study was to evaluate the multiplex polymerase chain reaction (PCR) for detection of Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomo-nas maltophilia from positive blood cultures for these pathogens isolated from children with hospi-tal-acquired sepsis compared to the conventional biochemical reactions for identification of these organisms. Methods: This study was a cross-sectional study performed on 100 isolates from pediatric blood cultures, including Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. The study also included 100 isolates of Escherichia coli as a negative control. All isolates were identified by API 20NE and the multiplex PCR with primers specific to the 3 tested bac-teria. Results: Multiplex PCR was positive in 96% of isolates and 4 isolates had negative results. False-positive results were reported with three E. coli strains. Multiplex PCR identified all the isolates of Acinetobacter baumannii, 29 isolates of Pseudomonas aeruginosa and 27 isolates of Stenotropho-monas maltophilia. The diagnostic value of the multiplex PCR compared to the biochemical identification revealed sensitivity 96.04%, specificity 96.9%, positive predictive value 97.00%, negative predictive value 96.00% and accuracy 96.50%. Conclusion: The present study highlights the diagnostic value of multiplex PCR to identify Acine-tobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from positive blood cultures. Multiplex PCR was sensitive, specific and accurate. The accuracy differs according to the organisms with 100% accuracy for Acinetobacter baumannii. © 2021 Bentham Science Publishers.
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页码：195 / 203
页数：8
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