Antioxidant hydrogel from poly(aspartic acid) and carboxymethylcellulose with quercetin loading as burn wound dressing

Susceptibility to infection and excessive accumulation of reactive oxygen species (ROS) are the greatest obstacles for burn wound healing. In this research, the 5-aminosalicylic acid (ASA) grafted poly(aspartic hydrazide) (PASH) was synthesized by successive ploysuccinimide (PSI) ring opening reaction and reacted with oxidized carboxymethyl cellulose (DCMC) to fabricate biodegradable hydrogel through Schiff-base cross-linking. Moreover, the hydrogel was loaded with quercetin (QT) to enhance its anti-inflammatory performance. The ASA moiety endowed the hydrogel with the free radical scavenging ability and mussel inspired tissue adhesion to maintain the healing bioenvironment of the wound. The loading of QT gave the hydrogel more phenolic hydroxy group and further enhanced the antioxidant capacity of the hydrogel. The in vitro experiment revealed the grafted ASA moiety and the loaded QT greatly enhanced the ROS elimination property and antibacterial property. Moreover, the QT loaded hydrogel accelerated the burn wound repairing rate in the in vivo mice model. Based on above result, the PASH/DCMC could act as a new platform for QT loading to promote the burn wound repairing.