Influence of flaxseed mucilage on the formation, composition, and properties of exopolysaccharides produced by different strains of lactic acid bacteria

Microorganisms produce a wide variety of polysaccharides. Due to biosafety considerations, lactic acid bacteria (LAB) are popular producers of exopolysaccharides (EPS) for various applications. In this study, we analyzed the composition and properties of EPS produced by L. delbrueckii ssp. bulgaricus and LAB from clover silage (L. fermentum AG8, L. plantarum AG9) after growth on Man, Rogosa, and Sharpe broth (MRS) and with the addition of flaxseed mucilage (FSM) using chromatography, microscopy, and biochemical methods. We found that adding 0.4 % FSM does not drastically alter the medium's rheology but substantially increases EPS yield (by 3.1 to 3.8 times) and modifies the composition and macrostructure of EPS, as well as changes the spatial organization of LAB cells. The presence of FSM led to the production of xylose- and glucose-enriched EPS, which also contained varying proportions of fucose, rhamnose, arabinose, mannose, glycosamines, and uronic acids, depending on the strain. Most EPS had a low molecular weight (up to 32 kDa), except for EPS produced by L. fermentum AG8 in FSM-containing medium, which had molecular weight of 163 kDa. All EPS exhibited a porous microstructure and demonstrated scavenging capacity for OH- and DPPH-radicals, as well as high levels of alpha-glucosidase and lipase inhibitory activities, even at low concentrations (<1 g<middle dot>L-1 of EPS). These characteristics make them promising for use in functional food production and medicine.