Factors affecting collagen gel contraction during osteogenic differentiation of mesenchymal stem cells

被引：0

作者：

Sule, Anupam Ashutosh ^{[1
,3
]}

Nana, Arvind ^{[2
]}

Dimitrijevich, S. Dan ^{[1
]}

机构：

[1] Department of Molecular Cardiovascular Physiology, Graduate School of Biomedical Sciences, University of North Texas Health Science Center, Fort Worth,TX, United States

[2] Department of Orthopeadic Surgery, Bone and Joint Center, University of North Texas Health Science Center, Fort Worth,TX, United States

[3] Department of Internal Medicine, St Joseph Mercy Oakland, Pontiac,MI, United States

来源：

Trends in Biomaterials and Artificial Organs | 2017年 / 31卷 / 03期

关键词：

Stem cells - Bone - Cell culture - Collagen;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Collagen Type I is the primary extracellular matrix protein of bone. Collagen hydrogels are often used as in vitro models for osteogenesis and are increasingly used matrix components of tissue engineered grafts. Factors affecting contraction of collagen hydrogels containing human mesenchymal stem cells differentiating in situ into osteoblasts were studied. High initial cell seeding density, use of late passage cells and more differentiated cells led to greater contraction of the collagen hydrogel. Lowering the serum concentration in the culture medium did not affect contraction but adversely affected proliferation. Telomerase immortalization of component cells decreased contraction of collagen hydrogel. Factors that resulted in minimal collage hydrogel contraction during osteogenic differentiation of mesenchymal stem cells, were achieved by the use of early passage, telomerase transfected cells, seeded at a low density and culturing the hydrogels under low serum conditions. Therefore this strategy provides a suitable model for in vitro studies of osteogenic differentiation and associated events. © 2017 Society for Biomaterials and Artificial Organs - India. All rights reserved.

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页码：81 / 89