Changes of Chemical Signals in Needles of Pinus massoniana with Different Resistance after Inoculation of Pine Wood Nematode

被引：0

作者：

Wei Y. ^{[1
]}

Liu Q. ^{[1
]}

Zhou Z. ^{[1
]}

Xu L. ^{[2
]}

Chen X. ^{[2
]}

Hao Y. ^{[2
]}

机构：

[1] Research Institute of Subtropical Forestry, CAF, Hangzhou

[2] Anhui Academy of Forestry, Hefei

来源：

Liu, Qinghua | 2018年 / Chinese Society of Forestry卷 / 54期

关键词：

Pine wood nematode; Pinus massoniana; Resistant; Signal molecular; Susceptible;

D O I：

10.11707/j.1001-7488.20180213

中图分类号：

学科分类号：

摘要：

Objective: This study was to observe the trends of chemical signals when the different resistant individuals of Pinus massoniana had been inoculated with pine wood nematodes, in order to provide the theoretic support for clarifying the resistant mechanism of infected trees of P. massoniana. Method: The high resistant and susceptible clones had been selected following a serious of breeding produce. The dynamic variation of signal moleculars including H2O2, O2, NO and Ca2+ was detected in needles of different resistant clones at 1, 3, 7, 15 and 30 d post inoculation (dpi). Result: The results showed that a large number of pine wood nematodes were detected in different parts of susceptible clones, while no nematodes were found in the high resistant clones. The H2O2 content significantly increased in different resistant clones at 1, 3 and 7 dpi. The H2O2 content in high resistant ones was returned to the health level after 15 dpi but the susceptible ones rose sustainably. As a result, the H2O2 content in susceptible clones was 1.77 times higher than that in resistant ones. The O2 content also significantly increased in both resistant and susceptible clones during 1 to 15 dpi. They had a same trends and no significantly differences. The high resistant clones reduced the O2 content at 30 dpi but the susceptible ones still increased it. There was no significant difference in NO content between high resistant clones and the control group during 1 to 15 dpi. It was significantly higher than control group at 30 dpi. However, the NO content in susceptible ones was significantly higher than that of the control group and high resistant ones at each time point. The variation trend of Ca2+content was similar between different resistant clones post inoculation. They both performed in an up-down-up-down trend. The Ca2+content of high resistant clones was significantly higher than that of control group and susceptible ones at 1 dpi and 15 dpi. However, the Ca2+content of high resistant clones maintained at an ordinary level at the other time points. The Ca2+content of susceptible clones was significantly higher than that of control group at each point. Conclusion: The content of H2O2, O2 and Ca2+ would increase rapidly when the pines were invaded by nematode. They could induce defensive response as signal moleculars. The high resistant clones had an effective way to control signal molecular content and finally returned to normal physiological metabolism. However, the susceptible ones could not control and the excessive ROS and Ca2+ would damage the plants. NO content was stable in the high resistant clones post inoculation. But it was accumulated at each time point in susceptible ones. The redundant NO is harmful for P. massoniana metabolism. © 2018, Editorial Department of Scientia Silvae Sinicae. All right reserved.

引用

页码：119 / 125

页数：6

共 33 条

[1] Chen B., Xian P.J., Qiao L., Et al., Research progress in sodium channel gene mutations and their association with insecticide resistance of insects, Acta Entomologica Sinica, 58, 10, pp. 1116-1125, (2015)
[2] He L.X., Wu X.Q., Ji J., Et al., Role of nitric oxide and nucleases in different pine species inoculated with a nematode (Bursaphelenchus xylophilus) in their resistance to the infection, Scientia Silvae Sinicae, 48, 1, pp. 109-114, (2012)
[3] He L.X., Wu X.Q., Yu L.Z., Et al., The difference of H2O2 and oxidative enzyme in the interaction of different resistance pines and Bursaphelenchus xylophilus, Journal of Nanjing Forestry University Natural Science Edition, 34, 6, pp. 13-17, (2010)
[4] He L.X., Wu X.Q., Yu L.Z., The relationship between difference of superoxide anion and lesion in the interaction of different varieties of pines and Bursaphelenchus xylophilus, Journal of Nanjing Forestry University: Natural Science Edition, 35, 2, pp. 25-30, (2011)
[5] Jin G., Ye J.R., Histopathological study on the nematode in seedling of Pinus thunbergii, Journal of Nanjing Forestry University: Natural Science Edition, 31, 4, pp. 115-120, (2007)
[6] Liu W., Yang B.J., Comparison on the shape of male spicules between Bursaphelenchus xylophilus and B. mucronatus, Forest Research, 8, 2, pp. 223-225, (1995)
[7] Long S.S., Roles of ROS, NO, cytoplasm Ca2+ and G-protein in the HR process of wheat to stripe rust, pp. 3-18, (2011)
[8] Wang A.G., Luo G.H., Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants, Plant Physiology Communications, 6, pp. 55-57, (1990)
[9] Xu F.Y., Ge M.H., Wang Q.M., Et al., Studies on the masson pine provenances resistance to pine wood nematode (PWN) disease in China, Journal of Nanjing Forestry University: Natural Science Edition, 22, 2, pp. 29-33, (1998)
[10] Xu L., Calcium signal transduction characteristics of CaM and CaMBP in interactions of Pinus massoniana and Bursaphelenchus xylophilus, pp. 15-28, (2013)

← 1 2 3 4 →