Photon counting statistical analysis for chemiluminescence detection

被引：0
|
作者：
Chen, Wen-Yang [1 ,3 ]
Zou, Ming-Qiang [1 ,2 ,3 ]
Liu, Feng [2 ,3 ]
Li, Jin-Feng [2 ]
机构：
[1] College of Instrumentation & Electrical Engineering, Jilin University, Changchun
[2] China Inspection Laboratory Technologies Co., Ltd., Beijing
[3] Chinese Academy of Inspection and Quarantine, Beijing
来源：
Faguang Xuebao/Chinese Journal of Luminescence | 2015年 / 36卷 / 07期
关键词：
Enhanced chemiluminescence immunoassay; Photon counting; Statistical analysis;
D O I：
10.3788/fgxb20153607.0854
中图分类号：
学科分类号：
摘要：
In enhanced chemiluminescence enzyme-linked immunoassay, major parameters such as detection result, efficiency of counting and S/N ratio, are related with not only photon counting devices but also statistics process of photon pulse emission in optical luminescence system. In this article, we measured the statistics characteristics of photon pulse in enhanced luminol chemiluminescence system and theoretically analyzed the design of photon counting detection device of chemiluminescence system by its statistics characteristics of its pulse emission time. The research suggests that the photon emission pulse intervals of chemiluminescence subject to Weibull distribution. ©, 2015, Chines Academy of Sciences. All right reserved.
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页码：854 / 860
页数：6
相关论文
共 22 条
  • [1] Wang J., Study on Immunoassay for Fumonisin B1, (2010)
  • [2] Zhou Y., Zhou T., Zhou R., Et al., Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody-coated gold nanoparticles as nanoprobes, J. Lumin., 29, 4, pp. 338-343, (2014)
  • [3] Fan F., Shen H.Y., Zhang G.J., Et al., Chemiluminescence immunoassay based on microfluidic chips for α-fetoprotein, Clin. Chim. Acta, 431, pp. 113-117, (2014)
  • [4] Ma L., Sun Y.Y., Kang X.J., Et al., Chemiluminescence development of nanobody-based flow injection chemiluminescence immunoassay for sensitive detection of human prealbumin, Biosens. Bioelectron., 61, pp. 165-171, (2014)
  • [5] Zhang H.S., Qi S.W., A rapid and sensitive chemiluminescence immunoassay based on magnetic particles for squamous cell carcinoma antigen in human serum, Clin. Chim. Acta, 412, 17, pp. 1572-1577, (2011)
  • [6] Ma X.X., Liu H.Z., Tang J.J., Et al., Comparison of three enzyme-linked immunosorbent assay methods for quantitative determination of ricin, Chin. J. Chem., 39, 5, pp. 685-689, (2011)
  • [7] Zhang J.R., Chen J., Liu Z.M., Progress of Electrochemical luminescence immunoassay technology, Chin. J. Chem., 38, 8, pp. 1219-1226, (2010)
  • [8] Zheng G.J., Fang L.Q., Chen H., Et al., Magnetic microparticle chemiluminescence immunoassay method for determination of estriol in human urine, Chin. J. Chem., 39, 1, pp. 62-66, (2011)
  • [9] Xue P., Zhang Z.J., Zhang X.X., Chemiluminescent analysis of carcinoembryonic antigen in serum samples, Chin. J. Chem., 39, 1, pp. 95-98, (2011)
  • [10] Shu L.T., Xu B.S., Zhang P.Y., Enhanced chemiluminescence enzyme immunoassay for determination of serum gastrin and establishment, Chin. J. Lab. Diagn., 10, 1, pp. 96-97, (2006)
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