Research on extraction and characterization of cellulase from commercial enzyme preparation

被引：0
|
作者：
Shu, Guowei [1 ]
Yang, Hui [1 ]
Chen, He [1 ]
Yang, Zhiwei [1 ]
机构：
[1] Shu, Guowei
[2] Yang, Hui
[3] Chen, He
[4] Yang, Zhiwei
来源：
Shu, G. | 1600年 / Maxwell Science Publications, 74, Kenelm Road,, B10, 9AJ, Birmingham, Small Heath, United Kingdom卷 / 05期
关键词：
Sulfur compounds - Bandpass filters - Extraction - Nitrogen compounds - Graphic methods;
D O I：
10.19026/ajfst.5.3170
中图分类号：
学科分类号：
摘要：
The extraction of cellulase from commercial enzyme preparation by ammonium sulfate precipitation and its enzymatic characterization were studied. The results were as follows: the conditions of precipitation was 60% saturation of ammonium sulfate, the recovery rate of carboxymethylcellulase (CMCase) and Filter Paper enzyme Activity (FPA) were 73.8 and 71.4%, respectively. The enzyme has optimal temperature of 55°C, optimal pH of 4.8, the ions of Co2+, Mn2+ in the buffer lowered the activity of CMCase, but the Cu2+ in low concentration activated the CMCase. The ions of Co2+, Mn2+ and Al3+ in the buffer lowered the activity of FPA, but the Cu2+and K+ in low concentration activated the FPA; Km and Vmax of the enzyme were 2.22×10-3g/mL and 1.11×104 U/h using Lineweaver-Burk graphic method, respectively. © Maxwell Scientific Organization, 2013.
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页码：839 / 842
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