Evaluation of commercial colilert18-quantitray® method by ISO techniques for enumeration and quantification of total coliforms and Escherichia coli in drinking-water of Buraidah, Saudi Arabia

被引：2
作者：
Al-Turki, A. [1 ]
El-Ziney, M.G. [2 ]
机构：
[1] Department of Plant Production and Protection, Qassim University, Buraidah
[2] Department of Food Science and Human Nutrition, College of Agriculture and Veterinary Medicine, Qassim University, Buraidah
来源：
Journal of Applied Sciences | 2009年 / 9卷 / 18期
关键词：
Coliert18® Qunati Tray; Coliforms; Drinking water; E; coli; Membrane filtration; Microbial indicators; Mutable-tube fermentation; Reference standard methods;
D O I：
10.3923/jas.2009.3357.3363
中图分类号：
学科分类号：
摘要：
In the present investigation, the Colilert18-QuantiTray® test is compared with ISO 9308-2:1999 Multiple-Tube Fermentation (MTF) and 9308-1:2000 Membrane Filtration (MF) methods for the detection of coliforms and E. coli in Buraidah drinking water. Regarding sensitivity and specificity of test methods using MTF as reference, the MF method showed a weak sensitivity, while the Colilert18® test showed the highest one. On the other hand, MF method exhibited a higher specificity compared with Colilert18® test. A moderately strong relationship among test methods of coliforms log transformed counts obtained with MF (cfu/100 mL) and Coliert18®/Quanti-Tray (MPN/100 mL) tests compared to MPN-MTF method was demonstrated. Regression analysis revealed the presence of strong linear correlations (p<0.01) between the three test methods and standard plate count of detecting the concentration of serially diluted E. coli LMG 2092 with high regression coefficients with MF and Colilert18® and lower value for MTF. The Colilert18® had E. coli detection down to 5 MPN/100 mL (≅ 0.05/1 mL). Results showed that Coliert18®/Quanti-Tray method is comparable with ISO methods further, the former method has more advantages such as higher sensitivity, maximum detection limit ability and time and labor saving. © 2009 Asian Network for Scientific Information.
引用
收藏
页码：3357 / 3363
页数：6
相关论文
共 18 条
  • [1] Andrew D.E., Lenore S.C., Eugene W.R., Arnold E.G., Mary A.H., Standard Methods for the Examination of Water and Wastewater: Centennial Edition, (2005)
  • [2] Brenner K.P., Rankin C.C., New screening test to determine the acceptability of 0.45-micron membrane filters for analysis of water, Appl. Environ. Microbiol., 56, pp. 54-64, (1990)
  • [3] Clark J.A., el Shaarawi A.H., Evaluation of commercial presence-absence test kits for detection of total coliforms, E. coli and other indicator bacteria, Appl. Environ. Microbiol., 59, pp. 380-388, (1993)
  • [4] Eckner K.F., Comparison of membrane filtration and multiple-tube fermentation by the Colilert and Enterolert methods for detection of waterborne bacteria, E. coli and enterococci used in drinking and bathing water quality monitoring in southern Sweden, Appl. Environ. Microbiol., 64, pp. 3079-3083, (1998)
  • [5] Edberg S.C., Allen M.J., Smith D.B., National field evaluation of a defined substrate method for the simultaneous enumeration of total coliforms and E. coli from drinking water: Comparison with the standard multiple tube fermentation method, Appl. Environ. Microbiol., 54, pp. 1595-1601, (1988)
  • [6] Edberg S.C., Allen M.J., Smith D.B., Comparison of coliert method and standard fecal methods, AWWA Research Foundation, (1994)
  • [7] Fricker E.J., Illingworth K.S., Fricker C.R., Use of two formulation of Colilert and Quanti-Tray for assessment of the bacteriological quality of water, Wat. Res., 31, pp. 2495-2499, (1997)
  • [8] Horman A., Hanninen M., Evaluation of lactose Tergitol-7, m-Endo LES, colilert18, ready coliform 100, water-check-100, 3M petrifilm EC and drycult coliform test methods for detection of total coliforms and E. coli, Wat. Res., 40, pp. 3249-3256, (2006)
  • [9] Water quality-Detection and Enumeration of Coliform Organisms, Thermotolerant Coliform Organisms and Presumptive Escherichia coli-Part 2: Multiple Tube (Most Probable Number) Method (ISO 9308-2: 1999), (1999)
  • [10] Water Quality-Detection and Enumeration of Escherichia coli and Coliform Bacteria, Part 1: Membrane Filtration Method (ISO 9308-1: 2000), (2000)
12