The Effect of Cell-wall Disruption Methods on Astaxanthin Extraction from Wet Algal Biomass of Haematococcus pluvialis

被引：0

作者：

Ren X. ^{[1
,2
]}

Chen L. ^{[2
]}

Liu T. ^{[2
]}

Li R. ^{[3
]}

机构：

[1] College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, Shandong

[2] Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong

[3] College of Agriculture, Shanxi Agricultural University, Jinzhong, 030801, Shanxi

来源：

Journal of Chinese Institute of Food Science and Technology | 2018年 / 18卷 / 12期

关键词：

Astaxanthin; Cell-wall disruption; Extraction efficiency; Fatty acid; Haematococcus pluvialis;

D O I：

10.16429/j.1009-7848.2018.12.014

中图分类号：

学科分类号：

摘要：

The effect of cell-wall disruption methods on astaxanthin extraction from wet algal biomass of Haematococcus pluvialis was investigated. The contents and compositions of total carotenoids and total lipid were compared, after various treatments of cell-wall disruption, included grinding, enzymatic hydrolysis, acid hydrolysis, alkaline hydrolysis and hot steam. The results showed that the carotenoids extraction efficiencies were significantly increased, but their compositions were untouched, after either acid hydrolysis or enzymatic hydrolysis. The alkaline hydrolysis caused the degradation of esterified astaxanthin and increased the content of free astaxanthin to 20 folds of the control. Meanwhile, the intracellular triglyceride also significantly degraded. Hot steam and grinding had no obvious effect on the contents and compositions of the astaxanthin and lipid. In conclusion, enzymatic hydrolysis and acid hydrolysis, which maintained the stability and biological activity of the astaxanthin and the fatty acid, were the most effective pretreatment methods for cyst cells of H. pluvialis to improve the extraction efficiency of the astaxanthin. © 2018, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：110 / 117

页数：7

共 23 条

[1]

Pan C.H., Chien Y.H., Effects of dietary supplementation of alga Haematococcus pluvialis (Flotow), synthetic astaxanthin and beta-carotene on survival, growth, and pigment distribution of red devil, Cichlasoma citrinellum (Gunther), Aquaculture Research, 40, 8, pp. 871-879, (2009)

[2]

Guerin M., Huntley M.E., Olaizola M., Haematococcus astaxanthin: applications for human health and nutrition, Trends in Biotechnology, 21, 5, pp. 210-216, (2003)

[3]

Lorenz R.T., Cysewski G.R., Commercial potential for Haematococcus microalgae as a natural source of astaxanthin, Trends in Biotechnology, 18, 4, pp. 160-167, (2000)

[4]

Kang C.D., Lee J.S., Park T.H., Et al., Comparison of heterotrophic and photoautotrophic induction on astaxanthin production by Haematococcus pluvialis, Applied Microbiology and Biotechnology, 68, 2, pp. 237-241, (2005)

[5]

Montsant A., Zarka A., Boussiba S., Presence of a nonhydrolyzable biopolymer in the cell call of vegetative cells and astaxanthin-rich cysts of Haematococcus pluvialis (Chlorophyceae), Marine Biotechnology, 3, 6, pp. 515-521, (2001)

[6]

Hagen C., Siegmund S., Braune W., Ultrastructural and chemical changes in the cell wall of Haematococcus pluvialis(Volvocales, Chlorophyta) during aplanospore formation, European Journal of Phycology, 37, 2, pp. 217-226, (2002)

[7]

Kim D.Y., Vijayan D., Praveenkumar R., Et al., Cell-wall disruption and lipid/astaxanthin extraction from microalgae: Chlorella, and Haematococcus, Bioresource Technology, 199, pp. 300-310, (2015)

[8]

Mendes-Pinto M.M., Raposo M.F.J., Bowen J., Et al., Evaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: effects on astaxanthin recovery and implications for bio-availability, Journal of Applied Phycology, 13, 1, pp. 19-24, (2001)

[9]

Sarada R., Vidhyavathi R., Usha D., Et al., An efficient method for extraction of astaxanthin from green alga Haematococcus pluvialis, Journal of Agricultural and Food Chemistry, 54, 20, pp. 7585-7588, (2006)

[10]

Ruen-Ngam D., Shotipruk A., Pavasant P., Comparison of extraction methods for recovery of astaxanthin from Haematococcus pluvialis, Separation Science and Technology, 46, 1, pp. 64-70, (2011)

← 1 2 3 →