In this letter, a novel noise-nulling superimposed pilots (NNSP) scheme for OFDM modulated Amplify- and-Forward relay networks is proposed, and a method to design superimposed training sequences is presented to estimate the channel of relay→destination link. By nulling the noising before superimposed pilots at the relay, the interference noise, introduced by the source → relay link, to the relay training sequences can be eliminated. Besides, the superimposed pilots designed here have perfect Peak-to- Average Ratio (PAR) performance and can to be applied in OFDM systems. Simulation results show that the NNSP yield better MSE performance than that without noise elimination. ©, 2015, Journal of Computational Information Systems. All right reserved.