Design of Raman spectroscopy measurement system based on shifted excitation method using two laser diodes with different wavelengths

被引：0

作者：

Wang X. ^{[1
]}

Wu J. ^{[1
]}

Fan X. ^{[1
]}

Xu Y. ^{[1
]}

Lu X. ^{[1
]}

Zuo Y. ^{[2
]}

机构：

[1] School of Aerospace Engineering, Xiamen University, Xiamen

[2] The 1st Metrology & Measurement Research Center of National Defense Science Industry of China, Changcheng Institute of Metrology & Measurement, Beijing

来源：

Hongwai yu Jiguang Gongcheng/Infrared and Laser Engineering | 2016年 / 45卷 / 01期

关键词：

Fluorescence suppression; Raman spectroscopy; Reconstruction algorithm; Shifted excitation;

D O I：

10.3788/IRLA201645.0106005

中图分类号：

学科分类号：

摘要：

Raman detection is often disturbed by fluorescence background, while Shifted Excitation Raman Difference Spectroscopy (SERDS) is an effective method for fluorescent suppression on Raman spectroscopy. Based on this method, a Raman spectroscopy measurement system was designed using two closely space fixed-wavelength laser diodes stabilized with the Volume Bragg Gratings. The output wavelength of laser diodes was stabilized by manipulating their power and temperature. The light path and circuit of spectral data acquisition were designed with back-thinned area array CCD with high sensitivity. Besides, the three different reconstruction algorithms of the difference spectrum, namely simple integration algorithm, simple integration with data interpolation algorithm and multiple energy constraint iterative deconvolution algorithm could be realized through software of this system. The Raman spectra of sesame oil of some brand in the presence of a highly fluorescent were measured with this system and then the spectra reconstructed with three different algorithms to process the difference spectrum respectively were compared. Experimental results show that the system designed in this paper can effectively reject the effect of fluorescence to the Raman spectroscopy measurement. © 2016, Editorial Board of Journal of Infrared and Laser Engineering. All right reserved.

引用

页数：6

共 16 条

[11]

Zhao J., Carrabba M.M., Allen F.S., Automated fluorescence rejection using shifted excitation Raman difference spectroscopy, Applied Spectroscopy, 56, 7, pp. 834-845, (2002)

[12]

Osticioli I., Zoppi A., Castellucci E.M., Fluorescence and Raman spectra on painting materials: reconstruction of spectra with mathematical methods, Journal of Raman Spectroscopy, 37, 10, pp. 974-980, (2006)

[13]

Osticioli I., Zoppi A., Castellucci E.M., Shift-excitation raman difference spectroscopy-difference deconvolution method for the luminescence background rejection from raman spectra of solid samples, Applied Spectroscopy, 61, 8, pp. 839-844, (2007)

[14]

Martins M.A.S., Ribeiro D.G., Pereira dos Santos E.A., Et al., Shifted-excitation Raman difference spectroscopy for in vitro and in vivo biological samples analysis, Biomedical Optics Express, 1, 2, pp. 617-626, (2010)

[15]

Cai Z., Zou W., Wu J., A dual-excitation difference technique for fluorescence rejection in Raman spectroscopy, 2011 International Symposium on Bioelectronics and Bioinformatics (ISBB), pp. 29-32, (2011)

[16]

Kasha M., Characterization of electronic transitions in complex molecules, Discuss Faraday Soc, 9, pp. 14-19, (1950)

← 1 2 →