The research on the material of hyaluronic acid, collagen and poly (ethylene glycol) diacrylate composite hydrogel

被引：0
|
作者：
Yuan, Jing [1 ]
Qiu, Lipeng [1 ]
Chen, Jinghua [1 ]
机构：
[1] School of Pharmaceutical Science, Jiangnan University, Wuxi
来源：
Gongneng Cailiao/Journal of Functional Materials | 2015年 / 46卷 / 15期
关键词：
Collagen; Hyaluronic acid; Hydrogel; Poly (ethylene glycol) diacrylate;
D O I：
10.3969/j.issn.1001-9731.2015.15.010
中图分类号：
学科分类号：
摘要：
Aiming at the brittleness of hyaluronic acid (HA) and collagen (Col) hydrogels in mechanical properties, this work was to prepare a composite hydrogel with biocompatibility and high strength by two-step cross-linking method. Hydrogels with different weight percent of HA and constant Col and poly (ethylene glycol) Diacrylate were obtained to tune the physical and chemical properties. Subsequently, L-lysine was used to modify the hydrogels. The SEM results show the honeycomb morphology and the pore size of the ternary hydrogel ranges from 80-180 μm. When the concentration of L-lysine is 40 mmol/L, the comperssion modulus reaches 414 kPa which is 19 times of non-modified hydrogels. Cell toxicity and implantation experiments indicate that the extracts of hydrogel has no obvious inhibitory effect on MC 3T3-E1 cells, and the slight immunological rejection on mice. This kind of composite hydrogel has great potential in cartilage repair. ©, 2015, Journal of Functional Materials. All right reserved.
引用
收藏
页码：15052 / 15057
页数：5
相关论文
共 20 条
  • [1] Griffith L.G., Tissue engineering-current challenges and expanding opportunities, Science, 295, 5557, pp. 1009-1014, (2002)
  • [2] Tibbitt M.W., Anseth K.S., Hydrogels as extracellular matrix mimics for 3D cell culture, Biotechnology and Bioengineering, 103, pp. 655-663, (2009)
  • [3] Yeh M.K., Liang Y., Cheng K., Et al., A novel cell support membrane for skin tissue engineering: Gelatin film cross-linked with 2-chloro-1-methylpyridinium iodide, Polymer, 52, pp. 996-1003, (2011)
  • [4] Brown E.M., Collagen-a natural scaffold for biology and engineering, Journal of the American Leather Chemists Association, 104, pp. 275-285, (2009)
  • [5] Yeung T., Georges P.C., Flanagan L.A., Et al., Effects of substrate stiffness on cell morphology, cytoskeletal structure and adhesion, Cell Motol Cytoskel, 60, 1, pp. 24-34, (2005)
  • [6] Liu L., Thompson A.Y., Heidaran M.A., Et al., An osteoconductive collagen hyaluronate matrix for bone regeneration, Biomaterials, 20, pp. 1097-1108, (1999)
  • [7] Shu X., Luo Y., Roberts M.C., Et al., Disulfide cross-linked hyaluronan hydrogels, Biomacromolecules, 3, pp. 1304-1311, (2002)
  • [8] Guo Y., Yuan T., Xiao Z., Et al., Hydrogels of collagen/chondroitin sulfate/hyaluronan interpenetrating polymer network for cartilage tissue engineering, Journal of Materials Science: Materials in Medicine, 23, pp. 2267-2279, (2012)
  • [9] Zhang H., Qadeer A., Chen W., In situ gelable interpenetrating double network hydrogel formulated from binary components: thiolated chitosan and oxidized dextran, Biomacromolecules, 12, pp. 1428-1437, (2011)
  • [10] Zhao Y., Nakajima T., Yang J., Et al., Proteoglycans and glycosaminoglycans improve toughness of biocompatible double network hysrogels, Advanced Materials, 26, pp. 436-442, (2014)
12