Characterization of the roles of DNA polymerases, clamp, and clamp loaders during s-phase progression and cell cycle regulation in the silkworm, bombyx mori

被引:0
|
作者
Hino M. [1 ]
Morokuma D. [1 ]
Mon H. [1 ]
Lee J.M. [1 ]
Kusakabe T. [1 ]
机构
[1] Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Hakozaki 6-10-1, Higashi-ku, 812-8581, Fukuoka
来源
Kusakabe, Takahiro (Kusakabe@agr.kyushu-u.ac.jp) | 1600年 / Japanese Society of Sericultural Sciences卷 / 85期
基金
日本学术振兴会;
关键词
Cell cycle; Clamp loaders; DNA polymerases; Replication factors; Silkworm;
D O I
10.11416/jibs.85.2_021
中图分类号
学科分类号
摘要
DNA replication is one of key event in cell-cycle progression, yet due to their importance and lethality, the chronological phenotypes of DNA synthesis machineries after the depletion of corresponding genes have proved difficult to study. In the present study, mRNAs for three DNA polymerases, a clamp, and three clamp loaders were gradually depleted from cultured silkworm cells by soaking RNAi. Interestingly, the depletion of these DNA synthesis factors had different effects on the cell growth rate and arrest of cell-cycle progression during time-lapse observation. The depletion of DNA polymerases immediately arrested the cell-cycle progression at the S phase, while that of PCNA, a DNA clamp, required more time to slow cell growth and finally induced apoptosis. Surprisingly, silkworm cells continued to undergo several rounds of cell division when the components of clamp loaders were knocked down. © 2016, Journal of Insect Biotechnology and Sericology. All rights reserved.
引用
收藏
页码:21 / 29
页数:8
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