Optimization of Lactobacillus fermentum DSM 20049 growth on whey and lupin based medium using response surface methodology

被引：0

作者：

Hanoune, Saida ^{[1
]}

Djeghri-Hocine, Baida ^{[1
,2
]}

Kassas, Zohra ^{[1
]}

Derradji, Zineb ^{[1
]}

Boudour, Aicha ^{[1
]}

Boukhemis, Messaouda ^{[1
]}

机构：

[1] Département de Biochimie, Faculté des Sciences, Laboratoire de Biochimie et Microbiologie Appliquée, Université Badji-Mokhtar, BP12, Annaba

[2] Ecole Nationale Supérieure des Sciences de la Mer et de L'aménagement du Littoral, Alger

来源：

Advance Journal of Food Science and Technology | 2015年 / 9卷 / 09期

关键词：

Growth medium; Lactobacillus fermentum DSM 20049; Lupin; Optimization; Whey;

D O I：

10.19026/ajfst.9.1759

中图分类号：

O62 [有机化学]; TQ [化学工业];

学科分类号：

070303 ; 0817 ; 081704 ;

摘要：

The aim of this study was to evaluate the potential use of whey components and lupin proteins as cheap nutrients in the formulation of optimum medium for lactic acid bacteria growth, for this purpose a Plackett and Burman design was used to screen variables (medium components and physical parameters) that have significant effects on the growth of Lactobacillus fermentum DSM 20049 in whey and lupin based medium. Statistical analysis of the results allowed selecting two factors having a significant positive effect on the bacterial growth (tween 80 and inoculum size). Central composite design was used to determine the optimum level of the selected factor. Under these optimized conditions, the composition of WLM (Whey and Lupin Medium) was the basic medium of whey and lupin supplemented with 1.56 mL/L of tween and inoculated at a rate of 5.25% with a maximum OD600 value of 6.88. Based on the comparison of Lactobacillus fermentum DSM 20049 growth on the formulated medium with that observed on MRS (standard laboratory medium), the growth of the strain in WLM is much higher than that observed in MRS medium with a maximum OD600 values of 7.04 on LWM and 4.08 on MRS medium, respectively and Lactobacillus fermentum DSM 20049 growth in the new medium was 72.54% higher than that on MRS. Thus, whey and lupin hydrolysates are good alternative sources of nitrogen that lead to a better growth of the strain and can be used for large-scale production. © 2015 Maxwell Scientific Publication Corp.

引用

页码：679 / 685

页数：6

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