Integrated comparative transcriptome and weighted gene co-expression network analysis provide valuable insights into the response mechanisms of Alisma orientale to cadmium stress

被引：2
作者：
Chang, Xiao [1 ]
Li, Jie [1 ]
Wei, Shengnan [1 ]
Ying, Jianan [1 ]
Nevill, Paul [2 ]
Qi, Zhechen [1 ]
Lu, Qixiang [1 ]
You, Zhengying [1 ]
机构：
[1] Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou
[2] Minesite Biodiversity Monitoring with eDNA Research Group, Trace and Environmental DNA (TrEnD) Laboratory, School of Molecular and Life Sciences, Curtin University, Bentley, WA
来源：
Science of the Total Environment | 2024年 / 957卷
基金：
中国国家自然科学基金;
关键词：
Alisma orientale; Cadmium stress; Physiological response; Transcription factor; Transcriptome;
D O I：
10.1016/j.scitotenv.2024.177401
中图分类号：
学科分类号：
摘要：
Cadmium (Cd) pollution poses a serious challenge to the quality and safe utilization of traditional Chinese medicine plants as well as human health. In this study, seedlings of the medicinal plant species Alisma orientale were subjected to different levels of Cd stress for 7 days to investigate the effects of Cd stress on its growth, physiological response, and transcriptome profiling. The results showed that under different Cd stress levels, the growth of A. orientale displayed an inverted U-shaped dose response curve as low-dose stimulation and high-dose inhibition. Cd was mainly enriched in roots in the high concentration treatment, and Cd content reached maximum under 200-μM Cd stress. Cd stress-induced indicators including H2O2 (14.1–228.8 % in leaves; 29.7–131.7 % in roots) and MDA (22.0–161.1 % in leaves; 30.0–201.1 % in roots) showed different degree of increase, except under 200-μM Cd stress, which had a slight decrease. Antioxidant enzyme system (SOD, POD and CAT) and nonenzymatic substances (SS, SP, total flavonoid and total polyphenols) played a key role to mitigate Cd toxic effects. Transcriptome analysis revealed 26,442 significantly differentially expressed genes, and plant-pathogen interactions and phenylpropanoid biosynthesis were identified as two key pathways. Through WGCNA joint analysis, the transcription factor genes R2R3-MYB (AoMYB12) and WRKY (AoWRKY5 and AoWRKY6) were identified as hub regulators of A. orientale in response to Cd stress. Our study provides experimental data on the effects of Cd stress on A. orientale growth and Cd accumulation in different plant parts, and investigated the transcriptomic and physio-biochemical features, advancing our understanding of the response and detoxification mechanisms of plants under Cd stress. © 2024 Elsevier B.V.
引用
收藏
相关论文
共 99 条
[1]  
Abubakar A.S., Feng X., Gao G., Yu C., Chen J., Chen K., Wang X., Mou P., Shao D., Chen P., Zhu A., Genome wide characterization of R2R3 MYB transcription factor from Apocynum venetum revealed potential stress tolerance and flavonoid biosynthesis genes, Genomics, 114, (2022)
[2]  
Agarwal P., Mitra M., Banerjee S., Roy S., MYB4 transcription factor, a member of R2R3-subfamily of MYB domain protein, regulates cadmium tolerance via enhanced protection against oxidative damage and increases expression of PCS1 and MT1C in Arabidopsis, Plant Sci., 297, (2020)
[3]  
Arduini I., Masoni A., Mariotti M., Ercoli L., Low cadmium application increase miscanthus growth and cadmium translocation, Environ. Exp. Bot., 52, pp. 89-100, (2004)
[4]  
Bailey T., Boden M., Buske F., Frith M., Grant C., Clementi L., Ren J., Li W., Noble W., MEME SUITE: tools for motif discovery and searching, Nucleic Acids Res., 37, pp. W202-W208, (2009)
[5]  
Beers F., Sizer W., A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase, J. Biol. Chem., 195, pp. 133-140, (1952)
[6]  
Bian S., Jin D., Sun G., Shan B., Zhou H., Wang J., Zhai L., Li X., Characterization of the soybean R2R3-MYB transcription factor GmMYB81 and its functional roles under abiotic stresses, Gene, 753, (2020)
[7]  
Cao X., Wang X., Tong W., Gurajala H.K., Lu M., Hamid Y., Feng Y., He Z., Yang X., Distribution, availability and translocation of heavy metals in soil-oilseed rape (Brassica napus L.) system related to soil properties, Environ. Pollut., 252, pp. 733-741, (2019)
[8]  
Chen C., Chen H., Zhang Y., Thomas H.R., Frank M.H., He Y., Xia R., TBtools: an integrative toolkit developed for interactive analyses of big biological data, Mol. Plant, 13, pp. 1194-1202, (2020)
[9]  
Chen S., Wang Q., Lu H., Li J., Yang D., Liu J., Yan C., Phenolic metabolism and related heavy metal tolerance mechanism in Kandelia obovata under Cd and Zn stress, Ecotoxicol. Environ. Saf., 169, pp. 134-143, (2019)
[10]  
Cheng Y., Wei H., Sun R., Tian Z., Zheng X., Rapid method for protein quantitation by Bradford assay after elimination of the interference of polysorbate 80, Anal. Biochem., 494, pp. 37-39, (2016)
12345678910