The influence of photosynthetic pigment chlorophyllin in light-driven LPMO system on the hydrolytic action of cellulases

被引:0
|
作者
Xin, Donglin [1 ]
Xing, Minyu [1 ]
Ran, Ganqiao [1 ]
Blossom, Benedikt M. [2 ,3 ]
机构
[1] Shaanxi Acad Sci, Bioagr Inst Shaanxi, Xian 710043, Shaanxi, Peoples R China
[2] Univ Copenhagen, Dept Geosci & Nat Resource Management, Rolighedsvej 23, DK-1958 Frederiksberg C, Denmark
[3] Bigelow Lab Ocean Sci, 60 Bigelow Dr, East Boothbay, ME 04544 USA
关键词
Cellulases; Cellulose; Lytic polysaccharide monooxygenase; TRICHODERMA-REESEI; MECHANISM;
D O I
10.1016/j.ijbiomac.2024.136714
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It has been demonstrated that LPMO reactions can be driven by light, using the photosynthetic pigment chlorophyllin to achieve efficient oxidative degradation of cellulose. However, the effect of chlorophyllin on cellulases remains unclear. This study discovered that chlorophyllin does not affect the hydrolytic activity of cellulases under dark conditions. However, under light exposure, chlorophyllin-derived reactive oxygen species (ROS) exhibit a strong inhibitory effect on cellulases. These ROS primarily inhibit the hydrolytic action of endoglucanase II (Cel5A) and cellobiohydrolase II (Cel6A), while the action of cellobiohydrolase I and beta-glucosidase remains unaffected. Scavenger studies revealed that singlet oxygen (1O2) is the key inhibitory ROS responsible for the inhibition of Cel5A and Cel6A. The removal of 1 O 2by sodium azide effectively mitigates this inhibition, increasing the conversion yield of cellulose to glucose by 25.9 % when using the light-driven LPMO system in conjunction with cellulases. This study provides new insights into the role of chlorophyllin-derived 1 O 2in hindering hydrolytic action of cellulases and demonstrates the successful mitigation of this inhibition by sodium azide, thereby enhancing the cooperative degradation of cellulose to glucose by the light-driven LPMO system and cellulases.
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页数:8
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