Lactic Acid production by Lactobacillus sp. from biodiesel derived raw glycerol

被引：0
作者：
Prada-Palomo, Yessica [1 ]
Romero-Vanegas, Marly [1 ]
Díaz-Ruíz, Pedro [1 ]
Molina-Velasco, Daniel [1 ]
Guzmán-Luna, Carolina [1 ]
机构：
[1] Universidad Industrial de Santander, Bucaramanga, Santander
来源：
CTyF - Ciencia, Tecnologia y Futuro | 2012年 / 5卷 / 01期
关键词：
Bioconversion; Biodiesel; Fermentation; Lactic Acid; Native microorganisms; Nuclear Magnetic Resonance; Raw glycerol;
D O I：
10.29047/01225383.213
中图分类号：
学科分类号：
摘要：
Lactic Acid (LA) is a versatile compound with extensive industrial usage. Lactic Acid Bacteria (LAB) are renowned for their capacity to ferment carbohydrates to LA. In the biodiesel industry, bioconversion of residual glycerol to monomers of biopolymers, such as LA, is an alternative for usage of this waste as raw material to produce a high value added biotechnological compound. This research evaluates LA production through fermentation processes using a Lactobacillus sp. native strain and raw glycerol from the biodiesel industry. Native strain was isolated from the processing of dairy, fruit, and vegetable products and through cultures in Man, Rogosa & Sharpe agar (MRS). After an adaptation phase, the fermentative capability of the strain was evaluated through chemical quantification of metabolites using Nuclear Magnetic Resonance (1H-NMR); fermentative potential was compared with a Lactobacillus sp. reference strain (ATCC 7469). The native strain Lactobacillus sp. CYP4 showed a higher bioconversion potential (LA concentration: 39.41 mM, conversion percentage: 39.27%, at 24 h, volumetric productivity: 1.28 g.L-1.h-1, yield: 0.71 g·g-1) from raw glycerol, when compared to the reference strain.
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页码：57 / 66
页数：9
相关论文
共 21 条
  • [1] Alvarez M.D.F., Medina R., Pasteris S.E., Strasser De Saad A.M., Sesma F., Glycerol metabolism of Lactobacillus rhamnosus ATCC 7469: Cloning and expression of two glycerol kinase genes, Journal of Molecular Microbiology and Biotechnology, 7, 4, pp. 170-181, (2004)
  • [2] Claisse O., Lonvaud-Funel A., Assimilation of glycerol by a strain of Lactobacillus collinoides isolated from cider, Food Microbiol., 17, 5, pp. 513-519, (2000)
  • [3] Da Silva G.P., Mack M., Contiero J., Glycerol: A promising and abundant carbon source for industrial microbiology, Biotechnol. Advances, 27, 1, pp. 30-39, (2009)
  • [4] Du W., Li W., Sun T., Chen X., Liu D., Perspectives for biotechnological production of biodiesel and impacts, Appl. Microbiol. Biotechnol., 79, 3, pp. 331-337, (2008)
  • [5] El-Ziney M.G., Arneborg N., Uyttendaele M., Debevere J., Jakobsen M., Characterization of growth and metabolite production of Lactobacillus reuteri during glucose/glycerol cofermentation in batch and continuous cultures, Biotechnology Letters, 20, 10, pp. 913-916, (1998)
  • [6] 2010-2011: EU Biodiesel Industry Production Forecasts Show First Decrease in 2011 Since Data Is Gathered, (2011)
  • [7] Sector de los Biocombustibles, (2012)
  • [8] Felis G.E., Dellaglio F., Torriani S., Chapter 15: Taxonomy of probiotic microorganisms, Prebiotics and Probiotics Science and Technology, pp. 591-637, (2009)
  • [9] Fischer G., Hizsnyik E., Prieler S., Shah M., Van Velthuizen H., Biofuels and Food Security, (2009)
  • [10] Garai-Ibabe G., Ibarburu I., Berregi I., Claisse O., Lonvaud-Funnel A., Irastorza A., Duenas M.T., Glycerol metabolism and bitterness producing lactic acid bacteria in cidermaking, Internat. J. Food Microbiol., 121, 3, pp. 253-261, (2008)
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