Effect of atmospheric-pressure helium plasma jet on cell culture medium

被引:0
作者
Takamura, Norimitsu [1 ]
Wang, Douyan [2 ]
Satoh, Takao [3 ]
Namihira, Takao [4 ]
Saitoh, Hisato [1 ]
Akiyama, Hidenori [1 ]
机构
[1] Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, 2-39-1, Kurokami
[2] Priority Organization for Innovation and Excellence, Kumamoto University, Kumamoto 860-8555, 2-39-1, Kurokami
[3] Food Processing Technology Labs., Kumamoto Industrial Research Institute, Kumamoto 860-8555, 3-11-38, Higashimachi
[4] Bioelectrics Research Center, Kumamoto University, Kumamoto 860-8555, 2-39-1, Kurokami
基金
日本学术振兴会;
关键词
Atmospheric-pressure plasma jet; Cell culture medium; Electric conductivity; HPLC; PH; Ultra-violet transmission;
D O I
10.1541/ieejfms.133.278
中图分类号
学科分类号
摘要
Atmospheric-pressure, non-thermal, plasma jet produced by using helium gas has been studied for various applications. Especially, plasma applications for biological treatment are attracted and researched all over the world. In this paper, various kinds of cell culture mediums were irradiated plasma jet, and its components were analyzed. The results showed that irradiating plasma jet to cell culture medium could cause to change its pH, electric conductivity and so on. These results suggest that irradiating plasma jet might cause to change cell form and to kill them. Moreover, plasma jet generate UV light, it can be cause to kill cells. It found that irradiation time of plasma jet is very important factor to treat cells. © 2013 The Institute of Electrical Engineers of Japan.
引用
收藏
页码:278 / 285+8
相关论文
共 15 条
[1]  
Tochikubo F., Fundamental of atmospheric pressure nonthermal plasma, IEEJ Journal, 126, 12, pp. 781-783, (2006)
[2]  
Oda T., Environmental protection by using discharge plasma, OYO BUTSURI, 72, 4, pp. 415-421, (2003)
[3]  
Kogoma M., Takaki K., Fujiwara T., Tochikubo F., Nozaki T., Okazaki K., Sawada Y., Itatani R., Generation of atmospheric-pressure glow discharge and its applications, JPFR Jpn, 79, 10, pp. 1000-1032, (2003)
[4]  
Kitano K., Hamaguchi S., Atmospheric-pressure lf microplasma jets, OYO BUTSURI, 77, 4, pp. 383-389, (2008)
[5]  
Matsumori M., Nakatsuka S., Kojio T., Mitsushima T., Morisako I., High-speed reduction of oxide film using atmospheric-pressure inductively coupled microplasma jet, Panasonic Technical Journal, 57, 2, pp. 19-23, (2011)
[6]  
Yamazaki H., Ohshima T., Tsubota Y., Maeda N., Nishimura Y., Fukushima S., Microbicidal activities of low frequency atmospheric pressure plasma jets on oral pathogens, Jpn. J Dent Mater, 29, 5, (2011)
[7]  
Nagatsu M., Ogino A., Evolution of plasma science and technology in bio-medical fields (toward new developments of plasma-bio interdisciplinary science), JPFR Jpn, 87, 10, pp. 715-720, (2011)
[8]  
Takamura N., Wang D., Seki D., Namihira T., Yano K., Saitoh H., Akiyama H., Protein transduction into eukaryotic cells using non-thermal plasma, Int. J. Plasma Environmental Sci. & Tech., 6, 1, pp. 59-62, (2012)
[9]  
Liu D.X., Bruggeman P., Iza F., Rong M.Z., Kong M.G., Global model of low-temperature atmospheric-pressure he + h2o plasmas, Plasma Sources Sci. Tech., 19, 2, (2010)
[10]  
Witte M.B., Barbul A., Role of nitric oxide in wound repair, Am J Surg, 183, 4, pp. 406-412, (2002)