Aqueous two-phase processes for separation and purification of R-phycoerythrin

被引：0
作者：
Zhao, Li [1 ,2 ]
Peng, Yiliang [2 ]
Fu, Qiushuang [2 ]
Cai, Weimin [1 ]
机构：
[1] School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin
[2] College of Life Sciences and Biotechnology, Harbin Normal University, Harbin
来源：
Harbin Gongye Daxue Xuebao/Journal of Harbin Institute of Technology | 2015年 / 47卷 / 06期
关键词：
Aqueous two-phase system; Potassium sodium tartrate; Purity; R-phycoerythrin;
D O I：
10.11918/j.issn.0367-6234.2015.06.010
中图分类号：
学科分类号：
摘要：
The phycoerythrin (R-PE) is a fluorescent protein, which has great commercial and industrial value. In order to obtain a high purity R-PE which above the food grade standard, the R-PE was separated and purified from the Porphyra haitanensis using aqueous two-phase system (ATPS). At the optimal conditions of polyethylene glycol (PEG) of 1500 in the ATPS (potassium sodium tartrate/PEG), pH of 8.06, the tie-line length (LTL) of 22.30% and volume ratio (Vr) of 0.12, purity of the isolated R-PE would increase from 0.43 (initial purity) to 1.47 after the first extraction, with a recovery rate of 84.42%. After the second ATPS extraction, achieved a purity and purification factor of 1.55 and 3.60, respectively. The isolated R-PE had a high biological activity. It was a successful purification process for R-PE for industrial application, with the advantages of high efficient and low cost. ©, 2015, Editorial Board of Journal Harbin Institute of Technology. All right reserved.
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页码：54 / 58
页数：4
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