Structural characterization and immunoregulatory mechanism of a low-molecular-weight polysaccharide from lotus root

被引:2
|
作者
Sun, Ying [1 ]
Zhang, Mengjie [1 ]
Jiang, Xueyu [1 ]
Peng, Kaidi [1 ]
Yi, Yang [1 ,4 ]
Meng, Yan [2 ,4 ]
Wang, Hongxun [3 ,4 ]
机构
[1] Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China
[2] Hubei Univ Chinese Med, Sch Pharm, Wuhan 430065, Peoples R China
[3] Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan 430023, Peoples R China
[4] 36 Huanhu Cent Rd, Wuhan 430048, Hubei Province, Peoples R China
基金
中国国家自然科学基金;
关键词
Lotus root polysaccharide; Purification; Structure characterization; Immunomodulatory activity; NF-KAPPA-B; EXPRESSION; PATHWAY; MAPK; TNF;
D O I
10.1016/j.ijbiomac.2024.135957
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The extraction of polysaccharide from lotus root was highly homogenized, and the structure of the polysaccharide was not clear. Herein, we report a hot water method combined with alpha-amylase that was applied to extract lotus root polysaccharide. After purified, a lotus root polysaccharide fraction LP60-a with high purity and low molecule weight was obtained. Systematic characterization of the structure of LP60-a was achieved by monosaccharide composition, methylation and NMR analysis, showing that LP60-a was composed of alpha-1,6glucan linked with a small amount of arabinogalactan. Conformational determination showed that LP60-a was a three-helix polysaccharide with random coil conformation. Furtherly, the immunomodulatory activities of LP60a were investigated in RAW264.7 macrophages. The data indicated that LP60-a could enhance the proliferation and phagocytosis of macrophages significantly, and induce the expression of NO and TNF-alpha in macrophages without causing inflammation. Moreover, LP60-a promoted the phosphorylation of MAPK p38 and JNK, as well as NF-kappa B p65, indicating that LP60-a could activate RAW264.7 cells through MAPK and NF-kappa B signaling pathways. In conclusion, the results imply that LP60-a could enhance the immune function of macrophages, presenting a possibility to play a role as an immunomodulatory agent in dietary supplements.
引用
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页数:9
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