Electro-optical countermeasures system includes four kinds of interference patterns that are the smokescreen passive, laser angle deception, laser blocking, and laser suppression. Based on these interference patterns, the photoelectric confrontation simulation system is established to confront the weather vane laser-guided bombs. The process of system simulation is described, and the mathematical models of laser propagation, smoke transmission, seeker signal processing, jamming calculation, bomb control and movement modules are mainly investigated, and then simulation experiments and data analysis are performed. According to the simulation results, the main factors that affect the interference effect of various interference manners are analyzed. Furthermore, influencing factors are divided into two types: transient and gradually-varied, and then the characteristics of the influence on interference effects are simultaneously analyzed. In addition, the simulation system has been applied in the semi-physical simulation project, and it can also provide both reference and basis for the analysis and evaluation of the photoelectric confrontation efficiency.