Differential proteomics analysis in sunflower (Helianthus annuus L.)

被引：0
作者：
Enghelab Street, Sefiddasht City, Iran [1 ]
机构：
[1] Enghelab Street, Sefiddasht City
来源：
Biotechnology | / 5卷 / 245-247期
关键词：
2-DE; Helianthus annuus; Molecular weights; Seed globulin; Sunflower;
D O I：
10.3923/biotech.2014.245.247
中图分类号：
学科分类号：
摘要：
The seed proteins of sunflower (Helianthus annuus) were qualitatively and quantitatively investigated. Qualitative studies were carried out using different electrophoretic techniques (2-DE). Analysis of the water extracted flour on SDS-PAGE gave eight major polypeptides clusters with MWs of lower than 14.4,14.4-18.4,18.4-25.0, 25-35, 35-45, 45-66.2, 66.2-116 and higher than 116 kDa. Second dimension gel showed that the polypeptides with MWs of 45-66.2 kDa are legumin-like proteins. Isoelectric points of the majority of the sunflower seed proteins were between 3 and 10. Mapping gels, however, showed that sunflower seed proteins were highly heterogeneous, especially the major bands. A quantative study indicated that the albumin, globulin, prolamin andglutelin fractions amounted 38.32, 39.04, 5.53 and 17.09%, respectively of the extracted proteins. © Science Alert. All Rights Reserved.
引用
收藏
页码：245 / 247
页数：2
相关论文
共 14 条
  • [1] Abasery A.W., Electrophoretic and Biochemical Studies on Oil Seed Proteins, (1992)
  • [2] Baudet I., Mosse I., Fractionation of sunflower seed proteins, J. Am. Oil Chem. Soc, 54, pp. A82-A86, (1977)
  • [3] Bradford M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72, pp. 248-254, (1976)
  • [4] Dalgalarrondo M., Raymond I., Azanza I.L., Sunflower seed proteins: Characterization and subunit composition of the globulin fraction, I. Exp. Bot., 35, pp. 1618-1628, (1984)
  • [5] Gheyasuddin S., Cater C M., Mattil K.F., Effect of several variables on the extractibility of sunflower seed proteins, J. Sci., 35, pp. 453-456, (1970)
  • [6] Hatje G., World Importance of Oilcrops and their Products, Oil Crops of the World, Their Breeding and Utilization, pp. 1-21, (1989)
  • [7] Loubert F.I., Sunflower seed proteins, Biochemica et Biophysica Acta, 16, pp. 520-523, (1955)
  • [8] Lennerts L., Oelschrote, Oelkuchen, Pflanzliche Oele undFette, Herkunft, Gewinnung, Verwendung, (1983)
  • [9] Plietz P., Damaschun G., Muller I.I., Schwenke K.D., The structure of IIS globulins from sunflower and rapeseed. A small-Angle X-ray scattering study, Eur. J. Biochem., 130, pp. 315-320, (1983)
  • [10] Rahma E.H., Rao M.S.N., Isolation and characterization of the major protein fraction of sunflower seeds, J. Agric. Foods Chem., 29, pp. 518-521, (1981)
12