Effect of Triton X-100 on Improving Urease Activity of Sporosarcina Pasteurii

Microbially induced carbonate precipitation (MlCP)technology is a new green reinforcement technology developed in recent years. How to improve the reinforcement efficiency of MlCP has always been a focus of scholars’attention. This work focuses on the principle of urease produced by Sporosarcina pasteurii and the characteristics of non-ionic surfactant. The experiment of improving MlCP efficiency with non-ionic surfactant Triton X-100 was designed. The results showed that:(Ⅰ)based on the results of single factor experiment,response surface experiment was designed to analyze and determine the optimal treatment conditions of the surfactant,namely,the dosage was 1. 37%,the treatment time was 5. 02 h,the treatment temperature was 34. 28 ℃,and the ratio of bacteria to cementation solution was 1 ∶ 2. 11;(Ⅱ)the calcium carbonate content and unconfined compressive strength of the solidified body increased by 25. 48% and 22. 83% respectively after the calcareous sand was reinforced by Sporosarcina pasteurii under the optimal conditions;(Ⅲ)the non-ionic surfactant Triton X-100 increases the permeability of the cell membrane of Sporosarcina pasteurii. On the one hand,more intracellular urease is released to the outside of the cell,while more urea enters the cell,which promotes the hydrolysis of urea and the formation rate of carbonate. On the other hand,the calcium carbonate gel structure generates with bacteria as the core more compact. Under the effect of these two aspects,the MlCP efficiency is effectively improved. Relevant ideas and research results can provide new reference and ideas for the improvement of MlCP reinforcement effect. © 2024 Cailiao Daobaoshe/ Materials Review. All rights reserved.