Development and application of a selenium speciation method in cattle feed and beef samples using HPLC-ICP-MS: evaluating the selenium metabolic process in cattle

被引：4

作者：

Oliveira, A. F. ^{[1
,2
,3
]}

Landero, J. ^{[3
]}

Kubachka, K. ^{[4
]}

Nogueira, A. R. A. ^{[2
]}

Zanetti, M. A. ^{[5
]}

Caruso, J. ^{[3
]}

机构：

[1] Univ Fed Sao Carlos, Dept Chem, Grp Appl Instrumental Anal, BR-13565905 Sao Carlos, SP, Brazil

[2] Embrapa Southeast Livestock, BR-13560970 Sao Carlos, SP, Brazil

[3] McMicken Coll Arts & Sci, Dept Chem, ML0172, Cincinnati, OH 45221 USA

[4] US FDA, Forens Chem Ctr, Cincinnati, OH USA

[5] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Anim Sci, BR-13630000 Pirassununga, SP, Brazil

来源：

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY | 2016年 / 31卷 / 04期

关键词：

INDUCTIVELY-COUPLED PLASMA; MASS-SPECTROMETRIC DETECTION; SIZE-EXCLUSION CHROMATOGRAPHY; YEAST; SUPPLEMENTATION; SELENOCYSTEINE; METHIONINE; TOXICITY;

D O I：

10.1039/c5ja00330j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Selenium (Se) is an essential element for mammals with diet being the major source of intake. For this work cattle feed was enriched with combinations of selenium enriched yeast, canola oil, and/or vitamin E in order to evaluate the accumulation and metabolism of selenium in beef cattle. A method to identify and/or quantify the selenium species: selenocystine (SeCys(2)), selenomethionine (SeMet), selenomethionine-Se-oxide (SeOMet), and inorganic selenium species, selenate (Se(VI)) and selenite (Se(IV)), was developed and applied to cattle feed and beef samples.

引用

页码：1034 / 1040

页数：7