Occurrence of multi-antibiotic resistant Pseudomonas spp. in drinking water produced from karstic hydrosystems

被引：50

作者：

Flores Ribeiro, Angela ^{[1
,2
]}

Bodilis, Josselin ^{[1
]}

Alonso, Lise ^{[1
]}

Buquet, Sylvaine ^{[1
]}

Feuilloley, Marc ^{[1
]}

Dupont, Jean-Paul ^{[2
]}

Pawlak, Barbara ^{[1
]}

机构：

[1] Laboratoire de Microbiologie-Signaux et Environnement (LMSM), EA 4312, Université de Rouen, 76821 Mont Saint Aignan, Place Emile Blondel, Bâtiment IRESE B, etage

[2] UMR 6143 Morphodynamique Continentale et CÔtière (M2C), Université de Rouen, 76821 Mont Saint Aignan, Place Emile Blondel, Bâtiment IRESE A

来源：

Science of the Total Environment | 2014年 / 490卷

关键词：

Antibiotic-resistant Pseudomonas spp; Drinking water; Plasmid;

D O I：

10.1016/j.scitotenv.2014.05.012

中图分类号：

学科分类号：

摘要：

Aquatic environments could play a role in the spread of antibiotic resistance genes by enabling antibiotic-resistant bacteria transferred through wastewater inputs to connect with autochthonous bacteria. Consequently, drinking water could be a potential pathway to humans and animals for antibiotic resistance genes. The aim of this study was to investigate occurrences of Escherichia coli and Pseudomonas spp. in drinking water produced from a karst, a vulnerable aquifer with frequent increases in water turbidity after rainfall events and run-offs. Water samples were collected throughout the system from the karstic springs to the drinking water tap during three non-turbid periods and two turbid events. E. coli densities in the springs were 10- to 1000-fold higher during the turbid events than during the non-turbid periods, indicating that, with increased turbidity, surface water had entered the karstic system and contaminated the spring water. However, no E. coli were isolated in the drinking water. In contrast, Pseudomonas spp. were isolated from the drinking water only during turbid events, while the densities in the springs were from 10- to 100-fold higher than in the non-turbid periods. All the 580 Pseudomonas spp. isolates obtained from the sampling periods were resistant (to between 1 and 10 antibiotics), with similar resistance patterns. Among all the Pseudomonas isolated throughout the drinking water production system, between 32% and 86% carried the major resistance pattern: ticarcillin, ticarcillin-clavulanic acid, cefsulodin, and/or aztreonam, and/or sulfamethoxazol-trimethoprim, and/or fosfomycin. Finally, 8 Pseudomonas spp. isolates, related to the Pseudomonas putida and Pseudomonas fluorescens species, were isolated from the drinking water. Thus, Pseudomonas could be involved in the dissemination of antibiotic resistance via drinking water during critical periods. © 2014 Elsevier B.V.

引用

页码：370 / 378

页数：8

共 59 条

[11]

Chee-Sanford J.C., Aminov R.I., Krapac I.J., Garrigues-Jeanjean N., Mackie R.I., Occurrence and diversity of tetracycline resistance genes in lagoons and groundwater underlying two swine production facilities, Appl Environ Microbiol, 67, pp. 1494-1502, (2001)

[12]

Chikwendu C.I., Amadi E.S., Obi R.K., Prevalence and antimicrobial resistance in Pseudomonas aeruginosa and Klebsiella pneumoniae isolates from non-clinical urine samples, N Y Sci J, 3, 11, pp. 194-200, (2010)

[13]

Dagert M., Ehrlich S.D., Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells, Gene, 6, 1, pp. 23-28, (1979)

[14]

De Vos D., Lim A., Pirnay J.-P., Struelens M., Vandenvelde C., Duinslaeger L., Et al., Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL, J Clin Microbiol, 35, 6, pp. 1295-1299, (1997)

[15]

Deredjian A., Colinon C., Brothier E., Favre-Bonte S., Cournoyer B., Nazaret S., Antibiotic and metal resistance among hospital and outdoor strains of Pseudomonas aeruginosa, Res Microbiol, 162, 7, pp. 689-700, (2011)

[16]

Figueira V., Vaz-Moreira I., Silva M., Manaia C.M., Diversity and antibiotic resistance of Aeromonas spp. in drinking and waste water treatment plants, Water Res, 45, 17, pp. 5599-5611, (2011)

[17]

Flores Ribeiro A., Laroche E., Hanin G., Fournier M., Quillet L., Dupont J.-P., Et al., Antibiotic-resistant Escherichia coli in karstic systems: a biological indicator of the origin of fecal contamination?, FEMS Microbiol Ecol, 81, 1, pp. 267-280, (2012)

[18]

Ford D.C., Williams P., Karst hydrogeology and Geomorphology, (2007)

[19]

Frost L.S., Leplae R., Summers A.O., Toussaint A., Mobile genetic elements: the agents of open source evolution, Nat Rev Microbiol, 3, 9, pp. 722-732, (2005)

[20]

Garcia-Armisen T., Vercammen K., Passerat J., Triest D., Servais P., Cornelis P., Antimicrobial resistance of heterotrophic bacteria in sewage-contaminated rivers, Water Res, 45, 2, pp. 788-796, (2011)

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