Preparation and characterization of magnetic cross-linked glucoamylase aggregates

被引：0
|
作者：
Zhang S. [1 ]
Chen G. [1 ,2 ,3 ]
Su P. [1 ]
机构：
[1] Department of Bioengineering and Biotechnology, College of Chemical Engineering, Huaqiao University, Xiamen, 361021, Fujian
[2] Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, 361021, Fujian
[3] Instrumental Analysis Center, Huaqiao University, Xiamen, 361021, Fujian
来源：
Huagong Xuebao/CIESC Journal | 2017年 / 68卷 / 07期
基金：
中国国家自然科学基金;
关键词：
Biocatalysis; Characterization of enzyme; Enzyme; Glucoamylase; Immobilization; Magnetic cross-linked enzyme aggregates; Preparation method;
D O I：
10.11949/j.issn.0438-1157.20161565
中图分类号：
学科分类号：
摘要：
The paper presents a method for preparing hybrid magnetic cross-linked enzyme aggregates (M-CLEAs) using carboxyl magnetic nanoparticles (MNP). The magnetic nanoparticle-enzyme complexes induced by electrostatic interaction between carboxyl group of magnetic particle and amino group of enzyme were separated from the solution under magnetic field, and then were cross-linked by glutaraldehyde. The traditional method of M-CLEAs preparation from amino-modified magnetic nanoparticles and the enzyme need the precipitant to prompt the separation of enzyme and magnetic nanoparticle from the solution. The method here proposed does not need the precipitant, thereof the process is greatly simplified. In this paper, the factors of cross-linking time, pH, enzyme concentration and concentration of glutaraldehyde in M-CLEAs preparation process were studied, and then the enzymatic properties of M-CLEAs were also investigated in detail. The results showed that the optimum conditions were as follows: enzyme concentration of 1 mg·ml-1, magnetic fluid concentration of 10 mg·ml-1, glutaraldehyde concentration of 0.25% and crosslinking reaction at pH 6.0 for 6 h. The final enzyme loading reached 80 mg·g-1 and the activity of M-CLEAs was 50 U·mg-1. The pH stability, thermostability and storage stability of the immobilized enzyme improved significantly, and M-CLEAs still remained nearly 60% of activity after 10 cycles. © All Right Reserved.
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页码：2763 / 2770
页数：7
相关论文
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