Effect of reclaimed water on bacterial community composition and function in urban river sediment

被引：0
作者：
Di Y.-M. [1 ,2 ]
Wang G.-X. [1 ,2 ]
Huang X.-R. [1 ,2 ]
Guo X.-Y. [1 ,2 ]
机构：
[1] College of Resources Environment and Tourism, Capital Normal University, Beijing
[2] Urban Environmental Processes and Digital Modeling Laboratory, Beijing
来源：
Guo, Xiao-Yu (xiaoyucnu@126.com) | 1600年 / Science Press卷 / 38期
关键词：
16S rRNA library; Bacterial community; Functional genes; Reclaimed water; T-RFLP; Urban river;
D O I：
10.13227/j.hjkx.201606089
中图分类号：
学科分类号：
摘要：
In order to study the effect of reclaimed water on bacterial community composition and function in urban river sediment, the changes of bacteria community diversity, composition and function in Mayu wetland upon the supply of reclaimed water were investigated by a range of sophisticated procedures, including Terminal Restriction Fragment Length Polymorphism(T-RFLP), 16S rRNA clone library technology, and Real-time Quantitative PCR Detecting System(qPCR).The results showed that carbon, nitrogen and phosphorus were major factors driving the variation of bacterial diversity and community structure in river sediment, and the bacteria were gradually recovered after purification in downstream under the effect of artificial wetland. In addition, the bacterial community in reclaimed water outfall was mainly constituted by β-Proteobacteria, δ-Proteobacteria, Bacteroidales and Cyanobacteriain, and ε-Proteobacteria, Chloroflexi and Spirochaetes were unique groups. Besides, the major biological geochemical cycle was nitrogen, carbon and phosphorus cycle in river sediment, which was closely related to functional genes. There were about 45.9% of the clones related to nitrogen cycle in reclaimed water outfall, such as Comamonas sp., higher than those of upstream and downstream (27.7% and 23.4%), 17.9% of the clones were closely related to the carbon cycle, such as Lysobacter sp., higher than those of upstream and downstream (14.4% and 12.9%). Furthermore, the trace of pathogenic bacteria and antibiotics in reclaimed water also changed the transformation pattern participating in carbon and nitrogen cycle, for example, Rhodocyclus sp. conducted nitrogen fixation by photosynthesis in reclaimed water outfall, whereas Burkholderia sp. fixes nitrogen by ways of plants symbiotic nitrogen fixation in upstream and downstream. This research provides theoretical reference for studies on remediation of reclaimed water supplying river by artificial wetland. © 2017, Science Press. All right reserved.
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页码：743 / 751
页数：8
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共 42 条
  • [31] Cao X.K., Yang Q., Hao C.B., Degradation kinetics of naphthalene by anaerobic sludge and analysis of the bacterial biodiversity, Environmental Science, 33, 10, pp. 3535-3541, (2012)
  • [32] Yan Q.Y., Yu Y.H., Feng W.S., Et al., Plankton community composition in the Three Gorges Reservoir Region revealed by PCR-DGGE and its relationships with environmental factors, Journal of Environmental Sciences, 20, 6, pp. 732-738, (2008)
  • [33] Chen M., Liu X.D., Wei L.W., Et al., Distribution of pollutants in water and silt along the upper Yongdinghe River, Rock and Mineral Analysis, 20, 2, (2001)
  • [34] Gong X., Wang J.H., Guan J.F., Et al., Impact of reclaimed water irrigation on soil chemical properties and culturable microorganisms, Environmental Science, 35, 9, pp. 3572-3579, (2014)
  • [35] Yu S., Wang M., Hong Y.W., Antibiotics in environmental matrices and their effects on microbial ecosystems, Acta Ecologica Sinica, 31, 15, pp. 4437-4446, (2011)
  • [36] Philippot L., Piutti S., Martin-Laurent F., Et al., Molecular analysis of the nitrate-reducing community from unplanted and maize-planted soils, Applied and Environmental Microbiology, 68, 12, pp. 6121-6128, (2002)
  • [37] Li L.Q., Wang D., Liu X.Y., Et al., Soil organic carbon fractions and microbial community and functions under changes in vegetation: a case of vegetation succession in karst forest, Environmental Earth Sciences, 71, 8, pp. 3727-3735, (2014)
  • [38] Zhang Y., Xie X., Jiao N., Et al., Diversity and distribution of amoA-type nitrifying and nirS-type denitrifying microbial communities in the Yangtze River estuary, Biogeosciences, 11, 8, pp. 2131-2145, (2014)
  • [39] Geldreich E.E., Litsky W., Fecal coliform and fecal streptococcus density relationships in waste discharges and receiving waters, CRC Critical Reviews in Environmental Control, 6, 4, pp. 349-369, (1976)
  • [40] Drury B., Rosi-Marshall E., Kelly J.J., Wastewater treatment effluent reduces the abundance and diversity of benthic bacterial communities in urban and suburban rivers, Applied and Environmental Microbiology, 79, 6, pp. 1897-1905, (2013)
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