Combination of Cell Growth Dependent and Independent Modes for Efficient L-Lactic Acid Production by Lactobacillus paracasei

被引：0

作者：

Zhang Y. ^{[1
,2
]}

Tian X. ^{[1
]}

Zhuang Y. ^{[1
,2
,3
]}

机构：

[1] School of Biotechnology, East China University of Science and Technology, Shanghai

[2] Demonstration Center of Experimental Teaching of Fermentation Engineering, East China University of Science and Technology, Shanghai

[3] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai

来源：

Science and Technology of Food Industry | 2021年 / 42卷 / 03期

关键词：

combination of cell growth dependence and independence; high density fermentation; lactic acid; Lactobacillus paracasei; resting cell;

D O I：

10.13386/j.issn1002-0306.2020040251

中图分类号：

学科分类号：

摘要：

In this study,on the basis of L-lactic acid production by Lactobacillus paracasei,a high cell density strategy with the combination of cell growth dependent and independent modes was developed through investigating the optimal initial glucose concentration and the metabolic capacity of resting cells. The results indicated that the cells grew well under 140 g/L of initial glucose concentration,and all the glucose could be rapidly exhausted to produce L-lactic acid. Moreover,the cells could still maintain high metabolic activity within 6~7 h after the end of fermentation. Finally,based on the above results,the L-lactic acid productivity of high cell density phase was enhanced by 1.4-fold compared with that of normal batch fermentation,and the L-lactic acid yield was also improved significantly to 0.985 g/g. This study extended the highly efficient production period so as to improve the production efficiency of L-lactic acid. Otherwise,the auxiliary time required in the fermentation process could be effectively reduced,including seed preparation and cell growth. Thus,it would be potentially applied for future industrial lactic acid production. © 2019 Food and Fermentation Industries. All rights reserved.

引用

页码：77 / 81and89

页数：8112

共 23 条

[1] Komesu A, Rocha De Oliveira J A, Da Silva Martins L H, Et al., Lactic acid production to purification:A review[J], BioResources, 12, 2, pp. 4364-4383, (2017)
[2] Ismail E, Amin M K, Francisco J B, Et al., Recent advacements in lactic acid production-a review[J], Food Res Int, 107, pp. 763-770, (2018)
[3] Arshadi M, Attard T M, Lukasik R M, Et al., Pre-treatment and extraction techniques for recovery of added value compounds from wastes throughout the agri-food chain[J], Green Chem, 18, 23, pp. 6160-6204, (2016)
[4] Upadhyaya B P, DeVeaux L C, Christopher L P., Metabolic engineering as a tool for enhanced lactic acid production[J], Trends Biotechnol, 32, 12, pp. 637-644, (2014)
[5] Luo S, Wu X, Zhu Y, Et al., Fermentative intensity of L-lactic acid production using self-immobilized pelletized Rhizopus oryzae[J], Afr J Biotechnol, 15, 21, pp. 974-979, (2016)
[6] Pimtong V, Ounaeb S, Thitiprasert S, Et al., Enhanced effectiveness of Rhizopus oryzae by immobilization in a static bed fermentor for L-lactic acid production[J], Process Biochem, 52, pp. 44-52, (2017)
[7] Yi X, Zhang P, Sun J, Et al., Engineering wildtype robust Pediococcus acidilactici strain for high titer L-and D-lactic acid production from corn stover feedstock[J], J Biotechnol, 217, pp. 112-121, (2016)
[8] Baek S H, Kwon E Y, Bae S J, Et al., Improvement of D-lactic acid production in Saccharomyces cerevisiae under acidic conditions by evolutionary and rational metabolic engineering[J], Biotechnol J, 12, 10, pp. 1-26, (2017)
[9] Abdel-Rahman M A, Tashiro Y, Sonomoto K., Recent advances in lactic acid production by microbial fermentation processes[J], Biotechnol Adv, 31, pp. 877-902, (2013)
[10] Tian X W, Wang Y H, Chu J, Et al., Enhanced L-lactic acidproduction in Lactobacillus paracasei by exogenous proline addition based on comparative metabolite profiling analysis[J], Appl Microb Biotechnol, 100, pp. 2301-2310, (2016)

← 1 2 3 →