Environmental concentrations of microplastic-induced gut microbiota and metabolite disruption in silkworm, Bombyx mori

被引：1

作者：

Zhang X. ^{[1
]}

Zheng W. ^{[1
]}

Shao W. ^{[1
]}

Yu W. ^{[1
]}

Yang Y. ^{[1
]}

Qin F. ^{[1
]}

Zhou W. ^{[2
]}

Gong C. ^{[3
]}

Hu X. ^{[3
]}

机构：

[1] School of Chemistry and Life Science, Suzhou University of Science and Technology, Suzhou

[2] Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou

[3] School of Biology & Basic Medical Science, Soochow University, Suzhou

来源：

Chemosphere | 2024年 / 358卷

基金：

中国国家自然科学基金;

关键词：

Gut metabolism; Gut microbiota perturbation; Microplastics; MPs exposure; Silkworm;

D O I：

10.1016/j.chemosphere.2024.142126

中图分类号：

学科分类号：

摘要：

Microplastics (MPs) existing extensively in various ecosystems can be ingested by marine organisms and enter the food chain, resulting the health risks from the presence of MPs in aquatic and terrestrial ecosystems. In the present study, an ideal model for Lepidoptera, the silkworm, Bombyx mori, was exposed to environmental concentrations (0.125 μg, 0.25 μg or 0.5 μg/diet) of MPs for 5 days, and the global changes in gut microbes and metabolites were subsequently examined via 16S rDNA sequencing and GC‒MS-based metabolomics. The results showed that MPs exposure did not seriously threaten survival but may regulate signaling pathways involved in development and cocoon production. MPs exposure induced gut microbiota perturbation according to the indices of α-diversity and β-diversity, and the functional prediction of the altered microbiome and associated metabolites demonstrated the potential roles of the altered microbiome following MPs exposure in the metabolic and physiological states of silkworm. The metabolites markedly altered following MPs exposure may play vital biological roles in energy metabolism, lipid metabolism, xenobiotic detoxiﬁcation and the immune system by directly or indirectly affecting the physiological state of silkworms. These findings contribute to assessing the health risks of MPs exposure in model insects and provide novel insight into the toxicity mechanism of MPs. © 2024 Elsevier Ltd

引用

共 28 条

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