Thyroid and sex hormone disrupting effects of DEHTP at different life stages of zebrafish (Danio rerio)

被引：2
作者：
Ihn Y. [1 ]
Cho Y. [1 ]
Lee Y. [1 ]
Seok H. [1 ]
Oh J.-S. [3 ]
Moon H.-B. [3 ]
Choi K. [1 ,2 ]
机构：
[1] Graduate School of Public Health, Seoul National University, Seoul
[2] Institute of Health and Environment, Seoul National University, Seoul
[3] Department of Marine Sciences and Convergence Engineering, College of Science and Convergence Technology, Hanyang University, Ansan
来源：
Chemosphere | 2024年 / 358卷
基金：
新加坡国家研究基金会;
关键词：
Adult male zebrafish; Alternative plasticizer; Embryo-larva; Endocrine disruption; Terephthalate;
D O I：
10.1016/j.chemosphere.2024.142105
中图分类号：
学科分类号：
摘要：
Di(2-ethylhexyl) terephthalate (DEHTP) is an alternative plasticizer widely used in numerous consumer products, replacing di(2-ethylhexyl) phthalate (DEHP). Hence, DEHTP has been frequently detected in the environment and humans. As a structural isomer and functional analog of DEHP, DEHTP is a suspected endocrine disruptor. Here, we evaluated thyroid-disrupting effects of DEHTP using embryo-larval and adult male zebrafish. We also investigated its sex hormone disruption potential in the adult zebrafish. After 5- and 7-days of exposure to DEHTP, significant increases in whole-body thyroid hormonal levels were observed in the larval fish. Down-regulation of several thyroid-regulating genes, including trh, tshβ, nis, and dio2, was observed, but only after 5-day exposure. Following a 21-day exposure, the adult male zebrafish exhibited a significant decrease in total triiodothyronine and an increase in thyroid-stimulating hormones. Potential changes in the deiodination of thyroid hormones, supported by the up-regulation of two deiodinases, dio1 and dio3a, along with the down-regulation of dio2, could explain the thyroid hormone changes in the adult zebrafish. Moreover, significant trends of decrease in estradiol and 11-ketotestosterone, along with increase of testosterone (T), were observed in the adult zebrafish. Up-regulation of several steroidogenic genes may explain elevated T, while exact mechanisms of action warrant further investigation. Our results demonstrate that DEHTP can cause disruptions of thyroid and sex hormones at different life stages in zebrafish. © 2024 Elsevier Ltd
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共 80 条
[1]  
Ankley G., Mihaich E., Stahl R., Tillitt D., Colborn T., McMaster S., Miller R., Bantle J., Campbell P., Denslow N., Dickerson R., Folmar L., Fry M., Giesy J., Gray L.E., Guiney P., Hutchinson T., Kennedy S., Kramer V., LeBlanc G., Mayes M., Nimrod A., Patino R., Peterson R., Purdy R., Ringer R., Thomas P., Touart L., Van der Kraak G., Zacharewski T., Overview of a workshop on screening methods for detecting potential (anti-) estrogenic/androgenic chemicals in wildlife, Environ. Toxicol. Chem.,
[2]  
Ashworth M.J., Chappell A., Ashmore E., Fowles J., Analysis and assessment of exposure to selected phthalates found in children's toys in christchurch, New Zealand, Int J Environ Res Public Health, 15, (2018)
[3]  
Bui T.T., Giovanoulis G., Cousins A.P., Magner J., Cousins I.T., de Wit C.A., Human exposure, hazard and risk of alternative plasticizers to phthalate esters, Sci. Total Environ., 541, pp. 451-467, (2016)
[4]  
Chang J., Wang M., Gui W., Zhao Y., Yu L., Zhu G., Changes in thyroid hormone levels during zebrafish development, Zoolog Sci, 29, pp. 181-184, (2012)
[5]  
Chen P.Y., Wang Q., Chen M., Yang J., Wang R.H., Zhong W.J., Zhu L.Y., Yang L.H., Antagonistic estrogenic effects displayed by bisphenol AF and perfluorooctanoic acid on zebrafish (Danio rerio) at an early developmental stage, Environ. Sci. Technol. Lett., 5, pp. 655-661, (2018)
[6]  
Chiang E.F., Yan Y.L., Guiguen Y., Postlethwait J., Chung B., Two Cyp19 (P450 aromatase) genes on duplicated zebrafish chromosomes are expressed in ovary or brain, Mol. Biol. Evol., 18, pp. 542-550, (2001)
[7]  
Chu S., Kwon B.R., Lee Y.M., Zoh K.D., Choi K., Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio), Chemosphere, 263, (2021)
[8]  
Corradetti B., Stronati A., Tosti L., Manicardi G., Carnevali O., Bizzaro D., Bis-(2-ethylexhyl) phthalate impairs spermatogenesis in zebrafish (Danio rerio), Reprod. Biol., 13, pp. 195-202, (2013)
[9]  
Toxicity review for di-2-ethylhexyl terephthalate (DEHT), (2018)
[10]  
Craig J.A., Ceballos D.M., Fruh V., Petropoulos Z.E., Allen J.G., Calafat A.M., Ospina M., Stapleton H.M., Hammel S., Gray R., Webster T.F., Exposure of nail salon workers to phthalates, di(2-ethylhexyl) terephthalate, and organophosphate esters: a pilot study, Environ. Sci. Technol., 53, pp. 14630-14637, (2019)
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