Approach model for simulation of the starch hydrolysis by α-Amylase and alcohol production from manioc root starch

被引：0

作者：

Curvelo-Santana, Jos Carlos ^{[1
]}

Librantz, Andr Felipe ^{[1
]}

Tambourgi, Elias ^{[2
]}

机构：

[1] Universidade Nove de Julho, Brazil

[2] Universidade Estadual de Campinas, Brazil

来源：

Chemical Product and Process Modeling | 2009年 / 4卷 / 04期

关键词：

Kinetics; -; Amylases; Hydrolysis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This work shows optimal conditions to use the amylases from A. niger in starch hydrolysis and alcohol production from manioc root starch. Bioreactors contained 10-20 g/L of manioc starch concentration, on 35-55C, and at pH 4.8; they were made for the evaluation of the effects of starch concentration and temperature on hydrolysis yield. New models for starch hydrolysis kinetics were compared for substratum dependence ([S]) to the hydrolysis time (t) and hydrolysis conversion rate (V) dependences with the substratum concentration ([S]). Results showed that exponential models fitted better than other models. The appropriate models were [S] = e-k.t and V = e(Vmax. [S]/KM), while k, Vmax and KM are the kinetic, maxim velocity and Michaelis-Menten constants, respectively. Models proposed in this work have been compared to experimental data, and it may be possible to simulate the starch hydrolysis process by amylases and its scale-up. Results showed that exponential models were more appropriate to use than Michaelis-Menten model for V values higher than 0.3 g/L.min. At a V range lower than 0.3 g/L.min, both models were satisfactory predictives. For starch hydrolysis, the exponential models perceived that, at optimal conditions, the mean yield was 70%. After fermentation, an alcohol yield above 45% was obtained (the theoretical is 51.1%). Copyright © 2009 The Berkeley Electronic Press. All rights reserved.

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