Antibiotic-Free High-Level <sc>l</sc>-Methionine Production in Engineered Escherichia coli

被引:4
作者
Wang, Lijuan [1 ,2 ]
Guo, Yingying [1 ,2 ]
Li, Mengyue [1 ,2 ]
Chen, Xiaowen [1 ,2 ]
Yang, Kun [1 ,2 ]
Liu, Zhiqiang [1 ,2 ]
Zheng, Yuguo [1 ,2 ]
机构
[1] Zhejiang Univ Technol, Natl & Local Joint Engn Res Ctr Biomfg Chiral Chem, Hangzhou 310014, Zhejiang, Peoples R China
[2] Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Key Lab Bioorgan Synth Zhejiang Prov, Hangzhou 310014, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
Escherichia coli; <sc>l</sc>-methionine; hok/sok; metabolic engineering; HOK MESSENGER-RNA; AMINO-ACIDS; THREONINE; MECHANISM;
D O I
10.1021/acs.jafc.4c06697
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
l-Methionine, a valuable sulfur-containing amino acid, holds great significance as a feed additive, nutraceutical, pharmaceutical, or even in the cosmetic industry. However, achieving efficient microbial production of l-methionine remains challenging due to its complex biosynthetic pathway and plasmid loss during fermentation. Herein, l-methionine biosynthesis was improved by enhancing succinyl-CoA supply, introducing a direct-sulfurylation pathway, and weakening the l-threonine branched pathway. The engineered strain produced 21.55 g/L l-methionine with a yield of 0.14 g/g glucose in a 5 L bioreactor. To eliminate the need for antibiotics and minimize plasmid loss, the hok/sok system was incorporated into the plasmid. The resulting plasmid pAm10 enabled strain M2 thrB(A1G) to produce 20.39 g/L of l-methionine without antibiotics in 5 L of fed-batch cultivation, a 42.58% increase compared to the control. This study highlights the potential of plasmid-based antibiotic-free fermentation for efficient and cost-effective production of l-methionine, as well as other amino acids or chemicals.
引用
收藏
页码:25791 / 25800
页数:10
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