In order to evaluate the co-fermentation performance of yeast fusant F11 and its tolerance to hemicellulose degradation products, the effects of initial pH, the ratio of glucose to xylose, temperature and inhibitor on the ethanol fermentation of F11 were investigated. The experimental results show that the optimal initial pH is from 4.0 to 7.0, ratio of glucose to xylose is 0 to 33%, and temperature is between 24 and 27 ℃. When the initial pH is 5.0, the rotating speed is 150 r/min, the temperature is 27 ℃, the initial inoculation amount is at 1 g/L, xylose and glucose concentration are 45 g/L and 15 g/L, respectively, and the ethanol concentration can attain to 28 g/L, which is about 91% of the theory yield. Moreover, it indicates that acetic acid (2.3 g/L) inhibites the ethanol fermentation intensely, and no ethanol has been produced within 24 hours due to its synergistic inhibitory effect with other degradation products like glucuronic acid (2.0 g/L), formic acid (0.9 g/L) and furfural (0.1 g/L). And all of these experimental data will provide valuable information for the cellulosic ethanol fermentation application. ©, 2015, Science Press. All right reserved.