Effects of fluvoxamine on nerve growth factor-induced neurite outgrowth inhibition by dexamethasone in PC12 cells

被引：7

作者：

Matsushima Y. ^{[1
,2
]}

Terada K. ^{[3
]}

Takata J. ^{[3
]}

Karube Y. ^{[3
]}

Kamei C. ^{[1
]}

Sugimoto Y. ^{[4
]}

机构：

[1] Faculty of Pharmaceutical Sciences, Department of Pharmacology, Yasuda Women’s University, Hiroshima

[2] Faculty of Pharmaceutical Sciences, Department of Kampo and Natural Product Chemistry, Yokohama University of Pharmacy, Yokohama

[3] Faculty of Pharmaceutical Sciences, Laboratory of Drug Design and Drug Delivery, Fukuoka University, Fukuoka

[4] Faculty of Pharmaceutical Sciences, Department of Pharmacology, Himeji Dokkyo University, Himeji

来源：

Bioscience, Biotechnology and Biochemistry | 2019年 / 83卷 / 04期

基金：

日本学术振兴会;

关键词：

Dexamethasone (DEX); Fluvoxamine; Nerve growth factor (NGF); Neurite outgrowth; Phosphorylated Akt;

D O I：

10.1080/09168451.2018.1553607

中图分类号：

O6 [化学]; TQ03 [化学反应过程]; TQ02 [化工过程（物理过程及物理化学过程）];

学科分类号：

0703 ; 081701 ; 081704 ;

摘要：

In the present study, we examined the effects of fluvoxamine on nerve growth factor (NGF)induced neurite outgrowth inhibition by dexamethasone (DEX) in PC12 cells. Fluvoxamine increased NGF-induced neurite outgrowth. Compared with co-treatment with NGF and fluvoxamine, p-Akt levels were higher than the values without fluvoxamine. The phosphorylated extracellular regulated kinase 1/2 levels were slightly increased by co-treatment with NGF and fluvoxamine. Fluvoxamine concentration-dependently improved NGF-induced neurite outgrowth inhibition by DEX. Fluvoxamine also improved the decrease in the NGF-induced p-Akt level caused by DEX. Interestingly, the sigma-1 receptor antagonist NE-100 blocked the improvement effects of fluvoxamine on NGF-induced neurite outgrowth inhibition by DEX. The selective sigma-1 receptor agonist PRE-084 also improved NGF-induced neurite outgrowth inhibition by DEX, which is blocked by NE-100. These results indicate that the improvement effects of fluvoxamine on NGF-induced neurite outgrowth inhibition by DEX may be attributable to the phosphorylation of Akt and the sigma-1 receptor. © 2018 Japan Society for Bioscience, Biotechnology, and Agrochemistry.

引用

页码：659 / 665

页数：6

共 25 条

[1]

Dinan T.G., Glucocorticoids and the genesis of depressive illness. A psychobiological model, Br J Psychiatry, 164, pp. 365-371, (1994)

[2]

McEwen B.S., Glucocorticoids, depression, and mood disorders: Structural remodeling in the brain, Metabolism, 54, pp. 20-23, (2005)

[3]

Sapolsky R.M., Glucocorticoids and hippocampal atrophy in neuropsychiatric disorders, Arch Gen Psychiatry, 57, pp. 925-935, (2000)

[4]

Holsboer F., Lauer C.J., Schreiber W., Et al., Altered hypothalamic-pituitary-adrenocortical regulation in healthy subjects at high familial risk for affective disorders, Neuroendocrinology, 62, pp. 340-347, (1995)

[5]

Liu H.H., Payne H.R., Wang B., Et al., Gender differences in response of hippocampus to chronic glucocorticoid stress: Role of glutamate receptors, J Neurosci Res, 83, pp. 775-786, (2006)

[6]

Sousa N., Lukoyanov N.V., Madeira M.D., Et al., Reorganization of the morphology of hippocampal neurites and synapses after stress-induced damage correlates with behavioral improvement, Neuroscience, 97, pp. 253-266, (2000)

[7]

Marques A.H., Silverman M.N., Sternberg E.M., Glucocorticoid dysregulations and their clinical correlates: From receptors to therapeutics, Immunology, 1179, pp. 1-18, (2009)

[8]

Kunugi H., Ida I., Owashi T., Et al., Assessment of the dexamethasone/CRH test as a state-ependent Mamrker for hypothalamic-pituitary-adrenal (HPA) xis Abanormalities in major depressive episode: A multicenter study, Neuropsychopharmacology, 31, pp. 212-220, (2005)

[9]

Wrobel A., Serefko A., Wlaz P., Et al., The depressogenic-like effect of acute and chronic treatment with dexamethasone and its influence on the activity of antidepressant drugs in the forced swim test in adult mice, Prog Neuropsychopharmacol Biol Psychiatry, 54, pp. 243-248, (2014)

[10]

Terada K., Kojima Y., Watanabe T., Et al., Inhibition of nerve growth factor-induced neurite outgrowth from PC12 cells by dexamethasone: Signaling pathways through the glucocorticoid receptor and phosphorylated Akt and ERK1/2, PLoS One, 9, (2014)

← 1 2 3 →